Strain Data Sheet



BRC No.RBRC01306
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hbegf<tm1.1Mek>/MekRbrc
Former Common nameHB-EGF Knockout mice(BL6)(HBdel#80/B6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003.The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice.
Strain descriptionHbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. This protein binds to and activates the EGF receptor. Homozygous mutant mice exhibit grossly enlarged cardiac valves and ventricular chambers. Most homozygous mice died in the first postnatal week. LacZ gene is expressed under the native HB-EGF promoter to examine the expression pattern of the HB-EGF histologically.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hbegfheparin-binding epidermal growth factor-like growth factor (mouse)18Hbegf<tm1.1Mek>targeted mutation 1.1, Eisuke Mekada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAphage P1 loxP site, E. coli lacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA
Research applicationCardiovascular Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
DepositorEisuke Mekada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Apr 2006
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data