Strain Data Sheet

RBRC01302

Information1

Image
BRC No.RBRC01302
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Srr2-Bgeo)1Okud/OkudRbrc
Former Common nameSRR2-βgeo transgenic mice
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Satoru Miyagi, Saitama Medical Scool at 2004. The transgene prasmid was injected into fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express lacZ in the ventricular layer of telencephalon in the embryo. This strain is used to trace neural stem cell/neural progenitor in vivo by X-gal staining.
Colony maintenance
ReferencesMol. Cell. Biol., 24, 4207-4220 (2004). 15121842

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox2SRY (sex determining region Y)-box 2UNSox2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UNherpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UNneo; neomycin;

Information2

Donor DNAmouse SRR2 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), beta-geo (E. coli neo, E. coli lacZ), SV40 intron plus polyA addition signal from pGL2 luciferase vector
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorAkihiko Okuda (Saitama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data