RBRC01297

Information1

Image
BRC No.RBRC01297
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameDBH-Cre/6-8
Former Common nameDBH-Cre/6-8
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express Cre recombinase gene under the control of the human dopamin beta hydroxylase (DBH) gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in typical noradrenergic and adrenergic cell groups as well as in transient catecholaminergic cell types. Line 6-8 (RBRC01297), Line 9-9 (RBRC01492).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesJ. Neurosci. Res., 78, 7-15 (2004). 15372500

Health Report

Examination Date / Room / Rack2020/03/30Room:4-ARack:L
2020/01/27Room:4-ARack:L
2019/11/25Room:4-ARack:L
2019/09/30Room:4-ARack:L
2019/07/29Room:4-ARack:L
2019/05/27Room:4-ARack:L
2019/03/25Room:4-ARack:L
2019/01/28Room:4-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcrehuman dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A

Information2

Donor DNAhuman dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, phage P1 Cre recombinase gene, rabbit beta-globin poly A, SV40 early gene poly A
Research applicationCell Biology Research
Cre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neursci. Res., 78, 7-15 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)
Mouse of the Month Jun 2005
Mouse of the Month June 2006

BRC mice in Publications

No Data