Strain Data Sheet

RBRC01295

Strain Information

Image
BRC No.RBRC01295
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAT-RhoA DN)4-18Koba/KobaRbrc
Former Common nameCAT-RhoA DN/4-18
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene containing CMV promoter and bovine Rho-A DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express a dominant-negative form for RhoA (RhoA DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. Line 4-18_RBRC01295, Line 4-19_RBRC01296.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]　　Tg mice are viable and fertile.
References
Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase.
Kobayashi K, Takahashi M, Matsushita N, Miyazaki J, Koike M, Yaginuma H, Osumi N, Kaibuchi K, Kobayashi K
J. Neurosci., 24, 3480-3488 (2004). 15071095

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rhoaras homolog gene family, member A (bovine)UNRhoa
catE. coli chloramphenicol acetyltransferase (CAT)UNCMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A
loxPphage P1 loxPUNloxP
loxPphage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • increased cell death(MP:0012556)

  • increased neuron apoptosis(MP:0003203)
    • Detailed phenotype data

    Ordering Information

    Donor DNACMV promoter, rabbit beta-globin intron, phage P1 loxP sites, E. coli chloramphenicol acetyltransferase gene, rabbit beta-globin poly A, bovine Rho-A DN cDNA, SV40 early gene poly A
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neursci., 24, 3480-3488 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Jun 2005

    BRC mice in Publications

    Negishi-Koga T, Shinohara M, Komatsu N, Bito H, Kodama T, Friedel RH, Takayanagi H.
    Suppression of bone formation by osteoclastic expression of semaphorin 4D.
    Nat Med 17(11) 1473-80(2011) 22019888