Strain Data Sheet

RBRC01295

Information1

Image
BRC No.RBRC01295
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAT-RhoA DN)4-18Koba/KobaRbrc
Former Common nameCAT-RhoA DN/4-18
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene containing CMV promoter and bovine Rho-A DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express a dominant-negative form for RhoA (RhoA DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. Line 4-18_RBRC01295, Line 4-19_RBRC01296.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]  Tg mice are viable and fertile.
ReferencesJ. Neurosci., 24, 3480-3488 (2004). 15071095

Health Report

Examination Date / Room / Rack2011/03/08Room:2-3Rack:C
2011/01/12Room:2-3Rack:C
2010/11/08Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rhoaras homolog gene family, member A (bovine)UNRhoa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNACMV promoter, rabbit beta-globin intron, phage P1 loxP sites, E. coli chloramphenicol acetyltransferase gene, rabbit beta-globin poly A, bovine Rho-A DN cDNA, SV40 early gene poly A
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neursci., 24, 3480-3488 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Jun 2005
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Negishi-Koga T, Shinohara M, Komatsu N, Bito H, Kodama T, Friedel RH, Takayanagi H.
Suppression of bone formation by osteoclastic expression of semaphorin 4D.
Nat. Med. (2011) 22019888