RBRC01269

Information1

Image
BRC No.RBRC01269
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(Cg)-Gnptab<tm1.1Zili>/SiecRbrc
Former Common nameStealth [lacZ/+]
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentTargeting of Stealth: 2001, Denis-Luc Ardiet, ISREC
Strain descriptionMice with a lacZ knock-in in the first major coding exon of Stealth. The lacZ ORF begins with the first start codon of that stealth exon. The loxP flanked neoR cassette was removed with help of a Cre deleter mouse of unknown hybrid genetic background.Purpose of that mouse strain is to analyze stealth function in mice, in particular for its role in development, innate immunity, and homeostasis of organ systems.
Colony maintenanceThis allele has been progressively backcrossed into a C57BL/6J (Harlan) background. The more prominent the C57BL/6J contribution became the more ataxic the more short lived mice homozygous for Stealth lacZ became. Beginning with the 4th backcross, the homozygotes died at birth or during the days before weaning.
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GnptabN-acetylglucosamine-1-phosphate transferase, alpha and beta subunits10Gnptab<tm1.1Zili>targeted mutation 1.1, Olav Zilian

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)10

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Information2

Donor DNAE. coli lacZ, phage P1 loxP site, SV40-3' UTR, mouse stealth genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Developmental Biology Research
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. To be cited is any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application.
DepositorOlav Zilian (Institut Suisse de Recherche Experimentale sur le Cancer (ISREC))
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

No Data