RBRC01268

Information1

Image
BRC No.RBRC01268
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Numb<tm1Zili> Numbl<tm1Zili>/SiecRbrc
Former Common nameNumb [loxP/loxP]; Numblike [loxP/loxP]
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentTargeting of Numb: 1997, Olav Zilian, ISRECTargeting of Numblike: 2001,Olav Zilian, ISREC
Strain descriptionCompound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase.
Colony maintenanceEfficient breeding is expected for these compound mice once homozygous for each conditionally targeted allele (Numb[lox / lox]; Numblike[lox / lox]) as they were efficient breeders when the compound homozygotes still carried FRTneoRFRT in both Numblike lox alleles.
ReferencesJ. Immunol., 178(11):6746-6751 (2007). 17513721
Curr. Biol., 11, 494-501 (2001). 11412999

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Numbnumb gene homolog (Drosophila)12Numb<tm1Zili>targeted mutation 1, Olav Zilian

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Numblnumb-like7Numbl<tm1Zili>targeted mutation 1, Olav Zilian

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Information2

Donor DNAphage P1 loxP, mouse Numb genomic DNA, mouse Numblike genomic DNA, yeast FRT (flipase recombination target) site
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application.
DepositorOlav Zilian (Institut Suisse de Recherche Experimentale sur le Cancer (ISREC))
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

No Data