Strain Data Sheet

RBRC01262

Information1

Image
BRC No.RBRC01262
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameC3.Cg-Mitf<mi-bw>/Rbrc
Former Common nameBlack-eyed white, Mitf<mi-bw> mutant mouse
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack eyed white [A/A B/B C/C mi<bw>/mi<bw>]
Strain developmentThe original mutant strain, C3HBSt carrying Mitf<mi-bw> allele was provided by Dr. Walter C. Quevedo Jr., Brown University to Tohoku University. The originals were backcrossed to C57BL/6J strain for 13 times. After several years, they were backcrossed to C3H/He strain because the homozygous mice became difficult to breed.
Strain descriptionC3;B6-Mitf<mi-bw>. The Mitf, microphthalmia-associated transcription factor plays a critical role in the development of melanocytes, mast cells, osteoclasts and pigmented epithelium. Homozygous mice for the Mitf<mi-bw> mutation display a white coat with black eyes. In this allele, a L1 element was inserted into intron 3. This strain is useful as an animal model for Waardenburg syndrome type 2 (WS2)
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygote shows white coat color with black eyes.
ReferencesHum. Mol. Genet., 8, 1431-1441 (1999). 10400990

Health Report

Examination Date / Room / Rack2008/02/18Room:3-CRack:F
2007/12/17Room:3-CRack:F
2007/10/15Room:3-CRack:F
2007/08/20Room:3-CRack:F
2007/06/18Room:3-CRack:F
2007/04/17Room:3-CRack:F
2007/04/16Room:3-CRack:F
2007/02/19Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mitfmicrophthalmia-associated transcription factor6Mitf<mi-bw>black eyed whiteblack eyed white, mi, wh

Information2

Donor DNA
Research applicationGenetics Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 8, 1431-1441 (1999).
DepositorHiroaki Yamamoto (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Dec 2006
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

BRC mice in Publications

Ohba K, Takeda K, Yamamoto H, Shibahara S.
Microphthalmia-associated transcription factor is expressed in projection neurons of the mouse olfactory bulb.
Genes Cells (2015) 26522736