BRC No.RBRC01262
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameC3.Cg-Mitf<mi-bw>/Rbrc
Former Common nameBlack-eyed white, Mitf<mi-bw> mutant mouse
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack eyed white [A/A B/B C/C mi<bw>/mi<bw>]
Strain developmentThe original mutant strain, C3HBSt carrying Mitf<mi-bw> allele was provided by Dr. Walter C. Quevedo Jr., Brown University to Tohoku University. The originals were backcrossed to C57BL/6J strain for 13 times. After several years, they were backcrossed to C3H/He strain because the homozygous mice became difficult to breed.
Strain descriptionC3;B6-Mitf<mi-bw>. The Mitf, microphthalmia-associated transcription factor plays a critical role in the development of melanocytes, mast cells, osteoclasts and pigmented epithelium. Homozygous mice for the Mitf<mi-bw> mutation display a white coat with black eyes. In this allele, a L1 element was inserted into intron 3. This strain is useful as an animal model for Waardenburg syndrome type 2 (WS2)
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygote shows white coat color with black eyes.
ReferencesHum. Mol. Genet., 8, 1431-1441 (1999). 10400990

Health Report

Examination Date / Room / Rack2008/02/18Room:3-CRack:F


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mitfmicrophthalmia-associated transcription factor6Mitf<mi-bw>black eyed white


Donor DNA
Research applicationGenetics Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 8, 1431-1441 (1999).
DepositorHiroaki Yamamoto (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Dec 2006

BRC mice in Publications

Ohba K, Takeda K, Yamamoto H, Shibahara S.
Microphthalmia-associated transcription factor is expressed in projection neurons of the mouse olfactory bulb.
Genes Cells (2015) 26522736