Strain Data Sheet
RBRC01260
Information1 | |
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| Image | |
| BRC No. | RBRC01260 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129(Cg)-Xist<tm2Sado> |
| Former Common name | Xist1loxGFP mice |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Takashi Sado, National Institute of Genetics at 2004. R1 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6. |
| Strain description | Xist gene knockout mice (Xist<1loxGFP>). Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). |
| Colony maintenance | Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] |
| References | |
Health Report | |
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| Examination Date / Room / Rack | 2022/02/21Room:2-検Rack:C 2021/11/22Room:2-検Rack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)X Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptorXSA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP |
Information2 | |
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| Donor DNA | SA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish GFP cDNA, poly A, phage P1 loxP site, mouse Xist genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
| Depositor | Takashi Sado (National Institute of Genetics) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Mouse of the Month Feb 2009 Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A. Sex-reversed somatic cell cloning in the mouse. J Reprod Dev 55(5) 566-9(2009) 19602850 Hirasawa R, Matoba S, Inoue K, Ogura A. Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos. PLoS One 8(10) e76422(2013) 24146866 Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A. Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. Science 330(6003) 496-9(2010) 20847234 |