BRC No.RBRC01260
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(Cg)-Xist<tm2Sado>
Former Common nameXist1loxGFP mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics at 2004. R1 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6.
Strain descriptionXist gene knockout mice (Xist<1loxGFP>). Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]

Health Report

Examination Date / Room / Rack2015/07/27Room:4-ARack:I


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Xistinactive X specific transcriptsXXist<tm2Sado>targeted mutation 2, Takashi Sado

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptorXSA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP


Donor DNAjellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP, SA, poly A, mouse Xist genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must inform the DEPOSITOR the research project using the
BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to
avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for
any patents with the results from these mice.
DepositorTakashi Sado (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Mouse of the Month Feb 2009

BRC mice in Publications

Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A.
Sex-Reversed Somatic Cell Cloning in the Mouse.
J. Reprod. Dev. (2009) 19602850

Hirasawa R, Matoba S, Inoue K, Ogura A.
Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos.
PLoS ONE 8(10) e76422(2013) 24146866

Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A.
Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer.
Science 330(6003) 496-9(2010) 20847234