RBRC01254

Information1

Image
BRC No.RBRC01254
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Cnot7<tm1Tno>/Rbrc
Former Common nameCnot7 Knockout mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Ryoji Yao, Tadashi Yamamoto, and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. An exon of Cnot7 subcloned the 18-kb genomic DNA fragment, was replaced by the insertion of a LacZ and a floxed neo cassette. The floxed neo cassette was excised in this strain. This strain originated on a B6;129 background.
Strain descriptionB6;129S4-Cnot7<tm1Tno>. Cnot7 gene (CCR4-NOT transcription complex. subunit 7) is essential for spermatogenesis. Cnot7 deficient male mice are sterile owing to oligo-astheno-teratozoospermia, impaired sperm motility, unsynchronized spermatid maturation, and Sertoli cell abnormalities. This knockout mouse strain is a powerful tool for understanding the mechanisms underlying spermatogenesis.
Colony maintenanceHeterozygote x Wild-typeHomozygous mutant males are sterile.
ReferencesNat. Genet., 36, 528-533 (2004). 15107851

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cnot7CCR4-NOT transcription complex, subunit 78Cnot7<tm1Tno>targeted mutation 1, Tetsuo Noda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)8nls

Information2

Donor DNAE. coli neo, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, mouse Cnot-7 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Phage P1 loxP
Research applicationCell Biology Research
Genetics Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Genetics, 36, 528-533 (2004).It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications.
The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
DepositorTetsuo Noda (Japanese Foundation for Cancer Research)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data