Strain Information | |
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Image | |
BRC No. | RBRC01252 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc |
Former Common name | FLP66, FLP66 mouse |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrlCrlj |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Tomonori Takeuchi and Masayoshi Mishina, Graduate School of Medicine, University of Tokyo in 1997. FLP recombinase rexpression vector pEF-NFLP was injected into the pronuclei of C57BL/6NCrj fertilized eggs. Three transgenic mouse lines (FLP22, FLP39, and FLP66) were produced. |
Strain description | These transgenic mice express a FLP recombinase gene under the control of the human polypeptide chian elongation factor 1 alpha (EF-1alpha) gene promoter. This can be used to make an inducible knock-out by corssing with the other genetically-engineeered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest. This strain is useful to remove the drug-resistance marker gene from mice manipulated for the conditional gene targeting using the Cre/loxP system. |
Colony maintenance | Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]. Tg homozygous and hemizygous mice are viable and fertile. |
References | Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
FLP | yeast flippase recombinase | UN | FLP | transgene insertion 66, Masayoshi Mishina | human elongation factor 1 alpha (EF-1 alpha) promoter | ||
nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | ||||
poly A | human granulocyte colony-stimulating factor gene polyAdenylation signal sequence | UN | poly A |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | human EF1-alpha promoter, SV40 large T antigen nuclear localization signal, yeast flp recombinase, human granulocyte colony-stimulating factor gene poly adenylation signal sequence |
Research application | Cell Biology Research FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 293, 953-957 (2002).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Masayoshi Mishina (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ichise T, Yoshida N, Ichise H. H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice. Development 137(6) 1003-13(2010) 20179099 |
Ichise H, Ichise T, Ohtani O, Yoshida N. Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice. Development 136(2) 191-5(2009) 19056831 |
Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 |
Fujii T, Ueda T, Nagata S, Fukunaga R. Essential role of p400/mDomino chromatin-remodeling ATPase in bone marrow hematopoiesis and cell-cycle progression. J Biol Chem 285(39) 30214-23(2010) 20610385 |