RBRC01230

Information1

Image
BRC No.RBRC01230
TypeTargeted Mutation and Chromosomal Aberration and F2 HybridCartagena
SpeciesMus musculus
Strain nameB6;129-Foxh1<tm1Hmd>/HmdRbrc
Former Common nameB6;129-FoxH1flox/flox, FASTflox/flox, B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearancewhite-bellied agouti [A<w>/? B/B C/C]
black [a/a B/B C/C]
Strain developmentDeveloped by Drs. Masamichi Yamamoto and Hiroshi Hamada at Osaka University in 2001. C57BL/6Cr and (129X1/SvJ x 129S1/Sv)F1-Kitl+ mixed background.
Strain descriptionThe transcription factor Foxh1 (horkhead box H1) gene mediates signaling by transforming growth factor-β, Activin, and Nodal. Foxh1 protein interacts with phospho-Smad2 to regulate specific downstream targets of these signals. Mutation in Foxh1 results in impaired orientation of anterior-posterior axis and various defects of anterior streak and node. The flox allele of this strain was generated by Flp-mediated deletion of the neo cassette located between frt sites from the original targeting vector which was designed to insert a combined loxP site and loxP-frt-neo-frt cassette. Conditional Foxh1 deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for the study of Foxh1 gene in vivo.
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygote mutant mice are normal and fertile.
ReferencesGenes Dev., 15, 1242-1256 (2001). 11358868

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FoxH1forkhead box H115FoxH1<tm1Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse FoxH1 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on
use of it from the DEPOSITOR.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data