Strain Data Sheet

RBRC01198

Information1

Image
BRC No.RBRC01198
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain name129.Cg-Tg(GOF18dPE/EGFP)48Ymat
Former Common name129;B6D2-Tg(GOF18δPE/EGFP)48, GOF18-delta PE-GFP.129
H-2 Haplotype
ES Cell line
Background strain129/Sv (hemizygote x 129/Sv)
AppearanceWhite-bellied gray
Strain developmentDeveloped by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).
Colony maintenanceCarrier x Noncarrier [or Crossing to 129<+Ter>/SvJcl]
ReferencesDev. Growth Differ., 41, 675-684 (1999). 10646797

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPGreen Fluorescent Protein (Aequorea victoria)UNEGFPOct 3 promoter

Information2

Donor DNAOct 3 promoter, Jellyfish GFP cDNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Purpose of study should be clarified.
DepositorAtsuo Ogura (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

Kajstura J, Rota M, Whang B, Cascapera S, Hosoda T, Bearzi C, Nurzynska D, Kasahara H, Zias E, Bonafé M, Nadal-Ginard B, Torella D, Nascimbene A, Quaini F, Urbanek K, Leri A, Anversa P.
Bone marrow cells differentiate in cardiac cell lineages after infarction independently of cell fusion.
Circ Res 96(1) 127-37(2005) 15569828

Miki H, Lee J, Inoue K, Ogonuki N, Noguchi Y, Mochida K, Kohda T, Nagashima H, Ishino F, Ogura A.
Microinsemination with first-wave round spermatids from immature male mice.
J Reprod Dev 50(1) 131-7(2004) 15007210