RBRC01198

Information1

Image
BRC No.RBRC01198
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain name129.Cg-Tg(GOF18dPE/EGFP)48Ymat
Former Common name129;B6D2-Tg(GOF18δPE/EGFP)48, GOF18-delta PE-GFP.129
H-2 Haplotype
ES Cell line
Background strain129/Sv (hemizygote x 129/Sv)
AppearanceWhite-bellied gray
Strain developmentDeveloped by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).
Strain descriptionOct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).
Colony maintenanceCarrier x Noncarrier [or Crossing to 129<+Ter>/SvJcl]
ReferencesDev. Growth Differ., 41, 675-684 (1999). 10646797

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNOct 3 promoter

Information2

Donor DNAOct 3 promoter, Jellyfish GFP cDNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Purpose of study should be clarified.
DepositorAtsuo Ogura (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

Jan KAJSTURA, Marcello ROTA, Brian WHANG, Stefano CASCAPERA, Toru HOSODA, Claudia BEARZI, Daria NURZYNSKA, Hideko KASAHARA, Elias ZIAS, Massimiliano BONAFE, Bernardo NADAL-GINARD, Daniele TORELLA, Angelo NASCIMBENE, Federico QUAINI, Konrad URBANEK, Annarosa LERI, Piero ANVERSA
Bone marrow cells differentiate in cardiac cell lineages after infarction independently of cell fusion.
Circ. Res. 96 127-137(2005) 15569828

Miki H, Lee J, Inoue K, Ogonuki N, Noguchi Y, Mochida K, Kohda T, Nagashima H, Ishino F, Ogura A.
Microinsemination with first-wave round spermatids from immature male mice.
J. Reprod. Dev. 50(1) 131-7(2004) 15007210