Strain Data Sheet


Strain Information

BRC No.RBRC01195
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S6-Grin2a<tm1Nak>/NakRbrc
Former Common nameNR2A KO, C57BL/6J-Grin2a tm
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C)]
Strain developmentDeveloped by Drs. H. Kadotani and S. Nakanishi, Kyoto University in 1996.
Strain descriptionB6.129S6-Grin2a<tm1Nak>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice are viable and fertile.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6J)
ReferencesJ. Neurosci., 16, 7859-7867 (1996). 8987814

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grin2aglutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2a<tm1Nak>targeted mutation 1, Shigetada Nakanishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)16neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse NMDA receptor NR2A subunit genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 16, 7859-7867 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigetada Nakanishi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Lu Y, Mu L, Elstrott J, Fu C, Sun C, Su T, Ma X, Yan J, Jiang H, Hanson JE, Geng Y, Chen Y.
Differential depletion of GluN2A induces heterogeneous schizophrenia-related phenotypes in mice.
EBioMedicine 102 105045(2024) 38471394

Gao X, Qiao Y, Jia B, Jing X, Cheng B, Wen L, Tan Q, Zhou Y, Zhu B, Qiao H.
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36.
Evid Based Complement Alternat Med 2012 438460(2012) 23118791

Su T, Lu Y, Fu C, Geng Y, Chen Y.
GluN2A mediates ketamine-induced rapid antidepressant-like responses.
Nat Neurosci (2023) 37709995