Strain Data Sheet

RBRC01195

Strain Information

Image
BRC No.RBRC01195
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S6-Grin2a<tm1Nak>/NakRbrc
Former Common nameNR2A KO, C57BL/6J-Grin2a tm
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C)]
Strain developmentDeveloped by Drs. H. Kadotani and S. Nakanishi, Kyoto University in 1996.
Strain descriptionB6.129S6-Grin2a<tm1Nak>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice are viable and fertile.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6J)
References
Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
Kadotani H, Hirano T, Masugi M, Nakamura K, Nakao K, Katsuki M, Nakanishi S
J. Neurosci., 16, 7859-7867 (1996). 8987814

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2aglutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2atargeted mutation 1, Shigetada Nakanishi
  • Landau-Kleffner syndrome(MedGEN)
    • neoneomycin resistance gene (E. coli)16herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • Landau-Kleffner syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal excitatory postsynaptic currents(MP:0002910)

  • abnormal miniature excitatory postsynaptic currents(MP:0004753)

  • behavior/neurological phenotype(MP:0005386)

  • decreased vertical activity(MP:0002757)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse NMDA receptor NR2A subunit genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 16, 7859-7867 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Lu Y, Mu L, Elstrott J, Fu C, Sun C, Su T, Ma X, Yan J, Jiang H, Hanson JE, Geng Y, Chen Y.
    Differential depletion of GluN2A induces heterogeneous schizophrenia-related phenotypes in mice.
    EBioMedicine 102 105045(2024) 38471394
    Gao X, Qiao Y, Jia B, Jing X, Cheng B, Wen L, Tan Q, Zhou Y, Zhu B, Qiao H.
    NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36.
    Evid Based Complement Alternat Med 2012 438460(2012) 23118791
    Su T, Lu Y, Fu C, Geng Y, Chen Y.
    GluN2A mediates ketamine-induced rapid antidepressant-like responses.
    Nat Neurosci (2023) 37709995