RBRC01190

Information1

Image
BRC No.RBRC01190
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Bcl11a<tm1Ngc>/Rbrc
Former Common nameEvi9/BclIIa Knockout mice
H-2 Haplotype
ES Cell lineCJ7 [129/SvlmJ]
Background strain
Appearancewhite-bellied agouti [A<w>/A<w> B/B C/C]
Strain developmentDeveloped by Drs. Pentao Liu and Neal G Copeland at NIH in 1999. A PGK-neo cassette replaced was transfered into CJ7 ES cells to replace exon 1 of Bcl11a gene.
Strain descriptionDeveloped by Drs. Pentao Liu and Neal G Copeland at NIH in 1999. A PGK-neo cassette replaced was transfered into CJ7 ES cells to replace exon 1 of Bcl11a gene.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote)Homozygous mutant mice die within 3-4hrs after birth.
ReferencesNat. Immunol., 4, 525-532 (2003). 12717432

Health Report

Examination Date / Room / Rack2012/02/06Room:3-2Rack:D
2011/12/05Room:3-2Rack:D
2011/10/03Room:3-2Rack:D
2011/08/08Room:3-2Rack:D
2011/06/06Room:3-2Rack:D
2011/04/04Room:3-2Rack:D
2011/02/07Room:3-2Rack:D
2010/12/06Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Bcl11aB-cell CLL/lymphoma 11A (zinc finger protein)11Bcl11a<tm1Ngc>targeted mutation 1, Neal G Copeland

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAadenovirus major late transcript splice acceptor (SA) sequence, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Bcl11a genomic DNA
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Immunol., 6, 525-532 (2003).
DepositorTakuro Nakamura (Japanese Foundation for Cancer Research)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data