Strain Data Sheet

RBRC01188

Strain Information

Image
BRC No.RBRC01188
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CTSG-NUP98/HOXA9)1589Tanak/TanakRbrc
Former Common namet(7;11)AML NUP98-HOXA9 transgenic (Tg) mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kakuro Nakamura, NIH in 1996. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J over 10 times.
Strain descriptionB6.Cg-Tg(CTSG-NUP98/HOXA9)1589Tanak. This transgenic mice express the chimeric NUP98-HOXA9 gene in the myeloid lineage. About 20% of mice with this transgene exhibit myeloproliferative disorder and acute myeloid leukemia (AML).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Identification of cooperative genes for NUP98-HOXA9 in myeloid leukemogenesis using a mouse model.
Masayuki Iwasaki, Takeshi Kuwata, Yukari Yamazaki, Nancy A Jenkins, Neal G Copeland, Motomi Osato, Yoshiaki Ito, Evert Kroon, Guy Sauvageau, Takuro Nakamura
Blood, 105, 784-793 (2005). 15454493

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CTSG Cathepsin G (human)UNCTSG
HOXA9 homeo box A9 (human)UNHOXA9
NUP98 nucleoporin 98 (human)UNNUP98 human Cathepsin G promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • increased leukemia incidence (MP:0002026)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman Cathepsin G genomic DNA, human NUP98-HOXA9 cDNA
    Research applicationCancer Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTakuro Nakamura (Japanese Foundation for Cancer Research)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Mar 2006

    BRC mice in Publications

    Funasaka T, Nakano H, Wu Y, Hashizume C, Gu L, Nakamura T, Wang W, Zhou P, Moore MA, Sato H, Wong RW.
    RNA export factor RAE1 contributes to NUP98-HOXA9-mediated leukemogenesis.
    Cell Cycle 10(9) 1456-67(2011) 21467841