RBRC01156

Information1

Image
BRC No.RBRC01156
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-Akt1/EGFP)3Tssk/TsskRbrc
Former Common nameA3, EGFP-Akt-3 transgenic mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development開発年:2002年開発者:佐々木雄彦先生機関名:東京都臨床医学総合研究所    秋田大学大学院医学系研究科C57BL/6J (クレア) の受精卵へDNA 注入。 6-7世代目
Strain description特性:セリン・スレオニンキナーゼ Akt の一部と緑色蛍光タンパク質の融合タンパク質を発現する。用途:Akt の細胞内動態及びイノシトールリン脂質の細胞内動態をモニターする。
Colony maintenance繁殖効率:A特になしホモ接合体の繁殖は可能だが繁殖効率が悪い。
ReferencesNature Cell Biol., 9, 36-44 (2007). 17173042

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Akt1thymoma viral proto-oncogene 1UNAkt1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Akt (serin threonine kinase) cDNA, Jellyfish GFP cDNA
Research applicationCancer Research
Cell Biology Research
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Metabolism Research
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Cell Biol., 9, 36-44 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, Dr. Sasaki T.
DepositorTakehiko Sasaki (Akita University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Nov 2007
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data