Strain Data Sheet



BRC No.RBRC01154
SpeciesMus musculus
Strain nameB6.Cg-Tg(Six3-cre)69Frty
Former Common nameTgN(Six3 Cre)69Frt; Six3Cre#69
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Yasuhide Furuta, University of Texas, M.D. Anderson Cancer Center. The Six3-cre transgene fragment was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6 (N5 times).
Strain descriptionTransgenic mice expressing Cre recombinase in a central region of the developing retina and widely in the ventral forebrain. Useful for tissue-specific gene activation/inactivation approaches. In particular, consequences of gene activation/inactivation in Cre-expressing cells within the retina can be assessed in direct comparisons to the surrounding non-Cre-expressing cells in an animal. Line 69 (RBRC01154), Line 22 (RBRC01153).
Colony maintenanceCarrier x Carrier
ReferencesGenesis, 26, 130-132 (2000). 10686607

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse Six3 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls


Donor DNAmouse six3 genomic DNA, phage P1 Cre recombinase, SV40-T-derived nuclear localization signal (NLS), bovine Growth Hormone poly-A signal
Research applicationCell Biology Research
Cre/loxP system
Genetics Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 26, 130-132 (2000).
DepositorYasuhide Furuta (The University of Texas M. D. Anderson Cancer Center)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month June 2006
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data