Strain Data Sheet

RBRC01151

Information1

Image
BRC No.RBRC01151
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S7-Hey1<tm1Kkb>/YsaRbrc
Former Common namehesr1, hairy and enhancer of split-related 1
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. H. Kokubo and R.L. Johnson at University of Texas M.D. Anderson Cancer Center in 1999. A loxP-flanked neo cassette was transferred into AB1 ES cells to replace the bHLH region, from exon 1 to exon 4. C57BL/6 and 129 mixed background.
Strain descriptionB6;129S7-Hey1<tm1Kkb>. Hey1 gene is a member of the hairy and enhancer of split related family of bHLH transcription factors, play a important roles in mediating Notch signaling in the developing cardiac and vascular systems.
Colony maintenanceSibling MatingHomozygous mutant mice are viable and fertile.
ReferencesDev Biol., 278, 301-309 (2005). 15680351

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hey1hairy and enhancer of split-related 13Hey1<tm1Kkb>targeted mutation 1, Hiroki KokuboHairy/E(spl)-related with YRPW motif 1, Herp2, hesr-1, HRT1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)3internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse hairy and enhancer of split-related 1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Harada Y, Yamada M, Imayoshi I, Kageyama R, Suzuki Y, Kuniya T, Furutachi S, Kawaguchi D, Gotoh Y.
Cell cycle arrest determines adult neural stem cell ontogeny by an embryonic Notch-nonoscillatory Hey1 module.
Nat Commun 12(1) 6562(2021) 34772946