RBRC01148

Information1

Image
BRC No.RBRC01148
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nanos1<tm1Ysa>/YsaRbrc
Former Common namenanos1 Knockout mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR MCH
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga, Natinal Institute of Genetics in 2000. LacZ and PGK-neo were inserted just before the translational start site of the nanos1 gene. The neo cassette was excised by crossing with CAG-Cre mice. Jcl:MCH (ICR) background.
Strain descriptionICR.Cg-Nanos1<tm1Ysa>/YsaRbrc. Nanos1, nanos homolog 1 (Dorosophila) is a nanos-related gene. Nanos encodes a RNA-binding protein containing a zinc-finger motif required for abdomen formation in Dorosophila. The nanos1 deficient mice are fertile and they show no detectable abnormality. Beta-gal activity indicates the nanos1 gene expressed in adult brain and gonad. This strain is useful for elucidation of the role of the nanos and the related genes.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
ReferencesMech. Dev., 120, 721-731(2003). 12834871

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nanos1nanos homolog 1 (Drosophila)19Nanos1<tm1Ysa>targeted mutation 1, Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)19

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Information2

Donor DNAmouse nanos1 genomic DNA, E. coli lacZ, phage P1 loxP, E. coli neo was deleted.
Research applicationDevelopmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mechanisms of Development, 120, 721-731 (2003).
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data