RBRC01145

Information1

Image
BRC No.RBRC01145
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc
Former Common nameMesp1-cre
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR MCH
AppearanceAgouti or black [A/? or a/a B/B Mesp1-Cre c/+ C]
albino [A/? or a/a B/B + c/+ C]
Strain developmentDeveloped by Yumiko Sga, National Institute of Health Sciences in 1998. A Cre gene and pgk-neo cassette were ligated in frame at a NcoI site located at the translational initiation site of Mesp1 gene. ICR mixed background.
Strain descriptionMesp1-Cre knock-in mice, in which Cre recombinase is expressed under the control of the endogenous promoter-enhancer. By crossing with a mouse strain with a reporter gene like a GFP and LacZ which is interrupted by a floxed CAT gene, it is possible to detect Mesp1-expressing cells. Mesp1 is a transcriptional factor temporarily expressing in endothelium of the blood vessel in a part of lateral plate mesoderm and heart cells, and is a determinant that is important for the formation of heart. This strain is useful for understanding of the mechanisms underlying cardiovascular development. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]. Homozygous mutant mice are embryonic lethal.
ReferencesDevelopment, 126, 3437-3447 (1999). 10393122

Health Report

Examination Date / Room / Rack2012/04/16Room:3-DRack:A
2012/02/29Room:3-DRack:A
2012/02/27Room:3-DRack:A
2012/02/27Room:3-DRack:A
2012/02/20Room:3-DRack:A
2012/02/08Room:3-DRack:A
2011/12/12Room:3-DRack:A
2011/12/12Room:3-DRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mesp1mesoderm posterior 17Mesp1<tm2(cre)Ysa>targeted mutation 2 (cre), Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase7creMesp1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Mesp1 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 126, 3437-3447 (1999).
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Feb 2006
Genetic Background
Saga Lab HP

BRC mice in Publications

MacDonald ST, Bamforth SD, Chen CM, Farthing CR, Franklyn A, Broadbent C, Schneider JE, Saga Y, Lewandoski M, Bhattacharya S.
Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
Cardiovasc. Res. 79(3) 448-57(2008) 18440989

Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM.
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Cell Stem Cell 3(1) 55-68(2008) 18593559

Thompson H, Ohazama A, Sharpe PT, Tucker AS.
The origin of the stapes and relationship to the otic capsule and oval window.
Dev. Dyn. 241(9) 1396-404(2012) 22778034

Rothova M, Thompson H, Lickert H, Tucker AS.
Lineage tracing of the endoderm during oral development.
Dev. Dyn. (2012) 22581563

Randall V, McCue K, Roberts C, Kyriakopoulou V, Beddow S, Barrett AN, Vitelli F, Prescott K, Shaw-Smith C, Devriendt K, Bosman E, Steffes G, Steel KP, Simrick S, Basson MA, Illingworth E, Scambler PJ.
Great vessel development requires biallelic expression of Chd7 and Tbx1 in pharyngeal ectoderm in mice.
J. Clin. Invest. 119(11) 510518(2009) 19855134

Dominguez-Frutos, E., Vendrell, V., Alvarez, Y., Zelarayan, L.C., Lopez-Hernandez, I., Ros, M. and Schimmang, T.
Tissue-specific requirements for FGF8 during early inner ear development.
Mech. Dev. 126(10) 873-81(2009) 19619645