Strain Data Sheet

RBRC01143

Information1

Image
BRC No.RBRC01143
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Dll1)1Ysa/YsaRbrc
Former Common nameCAG-CAT-Delta1
H-2 Haplotype
ES Cell line
Background strainICR MCH
Appearancealbino [c/c]
Strain developmentDeveloped by Dr. Yumiko Saga (2002). The transgene fragment was injected into the pronucleous of B6C3F1 fertilized eggs. This strain were maintained under ICR background.
Strain descriptionDll1, delta-like 1 (Drosophila) is one of Notch ligands. This transgenic mouse expresses the Dll1 gene after mating with a cre transgenic mouse. This strain is useful for elucidation of the Notch-signalling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenanceSibling Mating
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dll1delta-like 1 (Drosophila)UNDll1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
myc-tagmyc-epitope tagUNmyc-tag

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
signal pepUN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP, E. coli chloramphenicol acetyltransferase (CAT) gene, poly A, signal peptide, myc-tag, Delta1 cDNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F.
Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart.
Circ Res 106(3) 559-72(2010) 20007915