RBRC01142

Information1

Image
BRC No.RBRC01142
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Notch1)1Ysa/YsaRbrc
Former Common nameCAG-CAT-Notch1
H-2 Haplotype
ES Cell line
Background strainJcl:MCH(ICR)
Appearancealbino [c/c]
Strain developmentDeveloped by Dr. Yumiko Saga (2002). The transgene fragment was injected into the pronuclei of B6C3F1 fertilized eggs. This strain was maintained with ICR background.
Strain descriptionNotch1, Notch gene homolog 1 (Drosophila) is imortant in establishing metameric pattern during somitogenesis. This transgenic mouse expresses the active Notch1 gene after mating with a cre transgenic mouse. This strain are useful for elucidation of the Notch-signalling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenanceSibling Mating
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Notch1Notch gene homolog 1 (Drosophila)UNNotch1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, poly A, Notch1 intracellular domain cDNA, poly A
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F.
Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart.
Circ. Res. 106(3) 559-72(2010) 20007915