BRC No.RBRC01130
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ugt8a<tm1Pop>/Rbrc
Former Common nameCeramide galactosyltransferase (CGT)-deficient mice
H-2 Haplotype
ES Cell lineBK4 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Brain Popko, University of North Carolina in 1996.
Strain descriptionCGT-deficient mice fail to make galactolipid galactocerebroside and its sulfated derivative that are normal myelin constitutents. Mutant mice exhibit tremors, ataxia, progressive hindlib paralysis and vaculoe formation in ventral spinal cord due to abnormal myelin sheath.B6.129P2-Ugt8a<tm1Pop>. Ceramide galactosyltransferase, CGT is a key enzyme in the biosynthesis of galactocerebroside, GalC. Homozygous mutant mice for CGT gene show tremor, ataxia progressive hind limb paralysis and extensiv vacuolation of the ventral spinal cord.
Colony maintenanceSibling Mating
ReferencesJ. Biol. Chem., 275, 22623-22626 (2000). 10801776
J. Lipid Res., 41, 1237-1243 (2000). 10946011
蛋白質 核酸 酵素, 43,2318-2324 (1998). 9883654
蛋白質 核酸 酵素, 46, 45-54 (2001). 11193331
Cell, 86, 209-219 (1996). 8706126

Health Report

Examination Date / Room / Rack2009/04/21Room:3-CRack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ugt8UDP-glucuronosyltransferase 83Ugt8<tm1Pop>targeted mutation 1, Brian Popko

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)3


Donor DNAmouse Ceramide galactosyltransferase (CGT) genomic DNA, E. coli neo
Research applicationCell Biology Research
Neurobiology Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorBrian Popko (University of Chicago)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data