RBRC01123

Information1

Image
BRC No.RBRC01123
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain namehAG 2-5
Former Common namehAG 2-5
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Akiyoshi Fukamizu, University of Tsukuba in 1991. C57BL/6 background.
Strain descriptionHuman angiotensinogen gene transgenic mice with integration of 100 copies of a 14 kbp human DNA fragment containing the angiotensinogen gene (hAG 2-5 mice). The renin-angiotensin system (RAS) is one of the determinant factors in regulating blood pressure and sodium absorption. RAS has been shown to accelerate various diseases such as renal failure, atherosclerosis, cardiac hypertrophy, and pulmonary hypertension. The reaction between renin, produced in the kidney, and its substrate angiotensinogen, produced in the liver, is the initial and rate-limiting step in the enzymatic cascade that generates angiotensin I. Angiotensin-converting enzyme in the lung converts angiotensin I to the potent vasoconstrictor angiotensin II (Ang II). The depositor reported that overactivation of the RAS induces hypertension in the F1 generation obtained from breeding between a female carrying the human renin gene (hRN+/+) and a male possessing the human angiotensinogen gene (hAG+/+). Moreover, female hAG+/+ mice when bred with male hRN+/+ mice are hypertensive specifically in late pregnancy, suggesting possible implications for preeclampsia or pregnancy-induced hypertension, a life-threatening disorder for human mothers and fetuses. hRN+/+ and hANG+/+ mice provide the opportunity to understand the role of the RAS in hypertension and other common diseases. hRN8-12 mice (RBRC01122), hAG 2-5 mice (RBRC01123), hRN8-12 x hAG2-5)F1 mice (RBRC02432).
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) Homozygous mice are viable and fertile.
ReferencesBiochem. Biophys. Res. Commun., 180, 1103-1109 (1991). 1659395

Health Report

Examination Date / Room / Rack2020/06/15Room:3-2Rack:C
2020/06/08Room:3-2Rack:C
2020/04/06Room:3-2Rack:C
2020/02/03Room:3-2Rack:C
2019/12/02Room:3-2Rack:C
2019/11/14Room:3-2Rack:C
2019/11/14Room:3-2Rack:C
2019/10/16Room:3-2Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
AGTAngiotensinogen (human)UNAGT

Information2

Donor DNAhuman angiotensinogen genomic DNA
Research applicationCell Biology Research
Hematological Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochemical and Biophysical Research Communications, 180, 1103-1109 (1991).Within a period of 2 years after deposition of the material, user must contact bailor and notify him a content of the research. If the bailor judges that the research should be a collaborative work, then user must include bailor and/or developer's names as co-authors.
DepositorAkiyoshi Fukamizu (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)
Mouse of the Month May 2012
Systolic blood pressure
Genetic Background

BRC mice in Publications

Ogawa M, Suzuki JI, Takayama K, Senbonmatsu T, Hirata Y, Nagai R, Isobe M.
Impaired post-infarction cardiac remodeling in chronic kidney disease is due to excessive renin release.
Lab. Invest. (2012) 22986786