RBRC01122

Information1

Image
BRC No.RBRC01122
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain namehRN 8-12
Former Common namehRN 8-12
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Akiyoshi Fukamizu, University of Tsukuba in 1991. C57BL/6 background.
Strain descriptionHuman renin gene transgenic mice with integration of 10 copies of a 15 kbp human DNA fragment containing the renin gene (hRN 8-12 mice). The renin-angiotensin system (RAS) is one of the determinant factors in regulating blood pressure and sodium absorption. RAS has been shown to accelerate various diseases such as renal failure, atherosclerosis, cardiac hypertrophy, and pulmonary hypertension. The reaction between renin, produced in the kidney, and its substrate angiotensinogen, produced in the liver, is the initial and rate-limiting step in the enzymatic cascade that generates angiotensin I. Angiotensin-converting enzyme in the lung converts angiotensin I to the potent vasoconstrictor angiotensin II (Ang II). The depositor reported that overactivation of the RAS induces hypertension in the F1 generation obtained from breeding between a female carrying the human renin gene (hRN+/+) and a male possessing the human angiotensinogen gene (hAG+/+). Moreover, female hAG+/+ mice when bred with male hRN+/+ mice are hypertensive specifically in late pregnancy, suggesting possible implications for preeclampsia or pregnancy-induced hypertension, a life-threatening disorder for human mothers and fetuses. hRN+/+ and hANG+/+ mice provide the opportunity to understand the role of the RAS in hypertension and other common diseases. hRN8-12 mice (RBRC01122), hAG 2-5 mice (RBRC01123), hRN8-12 x hAG2-5)F1 mice (RBRC02432).
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) Homozygous mice are viable and fertile.
ReferencesBiochem. Biophys. Res. Commun., 165, 826-832 (1989). 2480785

Health Report

Examination Date / Room / Rack2020/04/06Room:3-2Rack:C
2020/02/03Room:3-2Rack:C
2019/12/02Room:3-2Rack:C
2019/11/14Room:3-2Rack:C
2019/11/14Room:3-2Rack:C
2019/10/16Room:3-2Rack:C
2019/08/05Room:3-2Rack:C
2019/06/03Room:3-2Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
REN1renin 1 structural (human)UNREN1

Information2

Donor DNAhuman renin1 genomic DNA (10 exons, 9 introns and native promoter)
Research applicationCell Biology Research
Hematological Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochemical and Biophysical Research Communications, 165, 826-832 (1989).
DepositorAkiyoshi Fukamizu (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)
Mouse of the Month May 2012
Systolic blood pressure
Genetic Background

BRC mice in Publications

Inaba S, Iwai M, Furuno M, Tomono Y, Kanno H, Senba I, Okayama H, Mogi M, Higaki J, Horiuchi M.
Continuous activation of renin-angiotensin system impairs cognitive function in renin/angiotensinogen transgenic mice.
Hypertension 53(2) 356-62(2009) 19047580

Falcao S, Bisotto S, Michel C, Lacasse AA, Vaillancourt C, Gutkowska J, Lavoie JL.
Exercise training can attenuate preeclampsia-like features in an animal model.
J. Hypertens. 28(12) 2446-53(2010) 20811291

Ogawa M, Suzuki JI, Takayama K, Senbonmatsu T, Hirata Y, Nagai R, Isobe M.
Impaired post-infarction cardiac remodeling in chronic kidney disease is due to excessive renin release.
Lab. Invest. (2012) 22986786

Inaba S, Iwai M, Tomono Y, Senba I, Furuno M, Kanno H, Okayama H, Mogi M, Higaki J, Horiuchi M.
Exaggeration of focal cerebral ischemia in transgenic mice carrying human Renin and human angiotensinogen genes.
Stroke 40(2) 597-603(2009) 19023100