RBRC01120

Information1

Image
BRC No.RBRC01120
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Agt<tm1Afu>/AfuRbrc
Former Common nameAGT-KO (BL/6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tanimoto K., University of Tsukuba in 2004. C57BL/6 background.
Strain descriptionB6.B6CB-Agt<tm1Afu>. Homozygous mutant mice do not produce angiotensinogen in the liver, resulting in the complete loss of plasma immunoreactive angiotensin I. The systolic blood pressure of the homozygous mutant is lower than wild-type, demonstrates an indispensable role for the renin-angiotensin system in maintaining blood pressure.
Colony maintenanceSibling Mating (Heterozygote x Wild-type)Homozygous mutant mice of C57BL/6 background show a low survivability. About 70 percent of homozygous mice
ReferencesJ. Biol. Chem., 269, 31334-31337 (1994). 7989296

Health Report

Examination Date / Room / Rack2006/08/07Room:3-8Rack:B
2006/06/06Room:3-8Rack:B
2006/04/04Room:3-8Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
AgtAngiotensinogen8Agt<tm1Afu>targeted mutation 1, Akiyoshi Fukamizu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8

Information2

Donor DNAmouse angiotensinogen genomic DNA, E. coli neo
Research applicationCell Biology Research
Hematological Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 269, 31334-31337 (1994).
DepositorAkiyoshi Fukamizu (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data