Strain Information | |
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Image | |
BRC No. | RBRC01120 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Agt<tm1Afu>/AfuRbrc |
Former Common name | AGT-KO (BL/6) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Tanimoto K., University of Tsukuba in 2004. C57BL/6 background. |
Strain description | B6.B6CB-Agt<tm1Afu>. Homozygous mutant mice do not produce angiotensinogen in the liver, resulting in the complete loss of plasma immunoreactive angiotensin I. The systolic blood pressure of the homozygous mutant is lower than wild-type, demonstrates an indispensable role for the renin-angiotensin system in maintaining blood pressure. |
Colony maintenance | Sibling Mating (Heterozygote x Wild-type)Homozygous mutant mice of C57BL/6 background show a low survivability. About 70 percent of homozygous mice |
References | Angiotensinogen-deficient mice with hypotension. Tanimoto K, Sugiyama F, Goto Y, Ishida J, Takimoto E, Yagami K, Fukamizu A, Murakami K J. Biol. Chem., 269, 31334-31337 (1994). 7989296 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Agt | angiotensinogen | 8 | Agt | targeted mutation 1, Akiyoshi Fukamizu | |||
neo | neomycin resistance gene (E. coli) | 8 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 38 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse angiotensinogen genomic DNA, E. coli neo |
Research application | Cell Biology Research Hematological Research covid19_onset |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 269, 31334-31337 (1994). |
Depositor | Akiyoshi Fukamizu (University of Tsukuba) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |