RBRC01119

Information1

Image
BRC No.RBRC01119
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Ren1<tm1Afu>/AfuRbrc
Former Common nameREN-KO (ICR)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR
Appearance
Strain developmentDeveloped by Dr. Keiji Tanimoto, University of Tsukuba in 2003. ICR background.
Strain descriptionICR.Cg-Ren1<tm1Afu>. Renin plays an essential role in the control of blood pressure through its specific leavage of angiotensinogen to generate angiotensin I. Homozygous mutant mice exhibit lowered blood pressure, increased urine and drinking volume, and altered renal morphology.
Colony maintenanceCross to ICR (Heterozygote x Crlj:CD1(ICR)) Homozygous mutant mice lowered blood pressure and die within a week after birth.
ReferencesJ. Biol. Chem., 275, 5-8 (2000). 10617578

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ren1renin 1 structural1Ren1<tm1Afu>targeted mutation 1, Akiyoshi Fukamizu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1

Information2

Donor DNAmouse renin 1 genomic DNA, E. coli neo, E. coli lacZ
Research applicationCell Biology Research
Hematological Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 275, 5-8 (2000).
DepositorAkiyoshi Fukamizu (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data