Strain Data Sheet



BRC No.RBRC01106
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Sema3a<tm1.2Tyag>/TyagRbrc
Former Common namesemaphorin III/D (conditional) Targeting mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainJcl:ICR
Appearancealbino [c/c]
Strain developmentDeveloped by Masahiko Taniguchi and Takeshi Yagi, National Institute for Physiological Sciences in 1997. TT2 ES cells were used. ICR mixed background.
Strain descriptionSemaphorin III/D (Sema3a) floxed mice Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to Jcl:ICR]
ReferencesNeuron, 19, 519-530 (1997). 9331345

Health Report

Examination Date / Room / Rack2022/07/11Room:3-ARack:C


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sema3asemaphorin III5Sema3a<tm1.2Tyag>targeted mutation 1.2, Takeshi Yagicoll-1, collapsin-1, Hsema-I, sema III, SEMA1, Semad, semaphorin III, SemD, SemD

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP


Donor DNAphage P1 loxP sites, mouse semaphorin 3A genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 519-530 (1997).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.
DepositorTakeshi Yagi (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Aug 2006
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Hayashi M, Nakashima T, Yoshimura N, Okamoto K, Tanaka S, Takayanagi H.
Autoregulation of Osteocyte Sema3A Orchestrates Estrogen Action and Counteracts Bone Aging.
Cell Metab 29(3) 627-637.e5(2019) 30661929

Fukuda T, Takeda S, Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C, Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H.
Sema3A regulates bone-mass accrual through sensory innervations.
Nature 497(7450) 490-3(2013) 23644455

Zhou J, Wen Y, She L, Sui YN, Liu L, Richards LJ, Poo MM.
Axon position within the corpus callosum determines contralateral cortical projection.
Proc Natl Acad Sci U S A 110(29) E2714-23(2013) 23812756

Imai T, Yamazaki T, Kobayakawa R, Kobayakawa K, Abe T, Suzuki M, Sakano H.
Pre-target axon sorting establishes the neural map topography.
Science 325(5940) 585-90(2009) 19589963

Shadrach JL, Pierchala BA.
Semaphorin3A Signaling Is Dispensable for Motor Axon Reinnervation of the Adult Neuromuscular Junction.
eNeuro 5(3) (2018) 29774231