Strain Data Sheet

RBRC01105

Information1

Image
BRC No.RBRC01105
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sema3a<tm1.3Tyag>/TyagRbrc
Former Common namesemaphorin III/D (lacZ) Targeting mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masahiko Taniguchi and Takeshi Yagi, National Institute for Physiological Sciences in 1997. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 5 times.
Strain descriptionSemaphorin III/D (Sema3a) knockout mice (lacZ reporter). Most of homozygous mice in C57BL/6 genetic background are lethal. About one-tenth of the homozygous mutant mice of ICR background survived (Depositor, personal communication).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6NCrlCrlj]
ReferencesNeuron, 19, 519-530 (1997). 9331345

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sema3asemaphorin III5Sema3a<tm1.3Tyag>targeted mutation 1.3, Takeshi Yagicoll-1, collapsin-1, Hsema-I, sema III, SEMA1, Semad, semaphorin III, SemD, SemD

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, E. coli lacZ, SV40 early pA signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse semaphorin 3A genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 519-530 (1997).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.
DepositorTakeshi Yagi (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Aug 2006
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Homman-Ludiye J, Bourne JA.
The guidance molecule Semaphorin3A is differentially involved in the arealization of the mouse and primate neocortex.
Cereb Cortex 24(11) 2884-98(2014) 23709645

Cioni JM, Telley L, Saywell V, Cadilhac C, Jourdan C, Huber AB, Huang JZ, Jahannault-Talignani C, Ango F.
SEMA3A signaling controls layer-specific interneuron branching in the cerebellum.
Curr Biol 23(10) 850-61(2013) 23602477

Zhou J, Wen Y, She L, Sui YN, Liu L, Richards LJ, Poo MM.
Axon position within the corpus callosum determines contralateral cortical projection.
Proc Natl Acad Sci U S A 110(29) E2714-23(2013) 23812756