RBRC01103

Information1

Image
BRC No.RBRC01103
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-Fyn-531)1Nko/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Nobuhiko Kojima, National Institute for Physiological Sciences, Japan at 1994. The FynB fragment mutated from TAT to TTT at codon 531 by site-directed mutagenesis under the control of the CaMKII alpha promoter was injected into the pronuclei of B6CBF1 fertilized egges. The transgenic mice were backcross to C57BL/6J mice for 26 generations.Generation:N23+N3
Strain descriptionB6.Cg-Tg(Camk2a-Fyn-531)1Nko/Rbrc. Transgenic mice overexpressing activated form of Fyn under the control of the calcium/calmodulin-dependent protein kinase II alpha promoter. The transgenic mutant Fyn is expressed at high levels in neurons of the forebrain. The transgenic mice exhibit seizure activity by stimulus of drug and light, accelerated kindling, and spontaneous death after weaning. The survival rate of these mice correlates inversely with the expression level of mutant Fyn. This strain is useful for elucidation of the role of Fyn tyrosine kinase in synaptic plasticity.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6JJcl)Reproductive performance is good.
ReferencesLearn. Mem., 5, 429-45 (1998). 10489260

Health Report

Examination Date / Room / Rack2011/03/08Room:2-3Rack:C
2011/01/12Room:2-3Rack:C
2010/11/08Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FynFyn proto-oncogeneUNFynmouse Camk2a promoter

Information2

Donor DNAmouse Fyn B (Y531F) cDNA, mouse CaMK IIα promoter
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Learning and Memory, 5, 429-445 (1998).
DepositorNobuhiko Kojima (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data