RBRC01102

Information1

Image
BRC No.RBRC01102
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-Fyn)1Kndl/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Nobuhiko Kojima, National Institute for Physiological Sciences, Japan at 1994. The FynB fragment including the CaMKII alpha promoter was injected into the pronuclei of B6CBF1 fertilized egges. The transgenic mice were backcross to C57BL/6J mice for 25 generations.Generation:N23+N2
Strain descriptionB6.Cg-Tg(Camk2a-Fyn)1Kndl/Rbrc. Transgenic mice overexpressing native Fyn under the control of the calcium/calmodulin-dependent protein kinase II alpha promoter. The transgenic Fyn is more expressed at high levels in the neurons of the adult forebrain than the neonatal one. The transgenic mice exhibit no obvious epileptic behavior, but the kindling of these mice is accelerated by once-daily stimulation of the amygdala. This strain is useful for elucidation of the role of Fyn tyrosine kinase in synaptic plasticity.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6JJcl)Reproductive performance is good.
ReferencesLearn. Mem., 5, 429-45 (1998). 10489260

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FynFyn proto-oncogeneUNFynmouse Camk2a promoter

Information2

Donor DNAmouse Fyn B cDNA, mouse CaMK IIα promoter
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Learning and Memory, 5, 429-445 (1998).
DepositorNobuhiko Kojima (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data