BRC No.RBRC01099
SpeciesMus musculus
Strain nameERAI mice
Former Common nameER stress-activated indicator (ERAI)
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Takao Iwawaki and Masayuki Miura, RIKEN Brain Science Institute. The 3.6-kb fragment of pCAX-F-XBP1deltaDBD-venus was injected into the pronuclei of C57BL/6 fertilized eggs. Transgenic mice were backcrossed to C57BL/6JJcl.
Strain descriptionERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and venus (a variant of green fluorescent protein) fusion gene under control of a CAG promoter. Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-venus fusion protein, which can be detected by fluorescence of the venus. This strain is useful to monitor physiological and pathological ER stress in vivo.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]. Tg mice are viable and fertile.
ReferencesNat. Med., 10, 98-102 (2004). 14702639

Health Report

Examination Date / Room / Rack2020/03/30Room:4-ARack:C


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)UNFLAGCMV-IE enhancer, chicken beta-actin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)UNVenus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
XBP1X-box binding protein 1 (human)UNXBP1


Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 intron and polyA signal, human XBP1 cDNA, Jellyfish venus (GFP) cDNA, FLAG tag
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Med., 10, 98-102 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. During 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR, and to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The material shall be used for teaching or not-for-profit research purposes only for the research described within this MTA. If there is intent to use the material for profit purpose, the recipient shall first discuss its intentions with RIKEN. The recipient shall agree to acknowledge RIKEN in all publications with regard to the material.
DepositorMasayuki Miura (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Mar 2007
Genetic Background

BRC mice in Publications

Norlin S, Parekh VS, Naredi P, Edlund H.
Asna1/TRC40 controls beta cell function and ER homeostasis by ensuring retrograde transport.
Diabetes (2015) 26438609

Wang X, Wang G, Kunte M, Shinde V, Gorbatyuk M.
Modulation of Angiogenesis by Genetic Manipulation of ATF4 in a Mouse Modulation of Angiogenesis by Genetic Manipulation.
Invest. Ophthalmol. Vis. Sci. 54(9) 5995-6002(2013) 23942974

Kunte MM, Choudhury S, Manheim JF, Shinde VM, Miura M, Chiodo VA, Hauswirth WW, Gorbatyuk OS, Gorbatyuk MS.
ER stress is Involved in T17M Rhodopsin-Induced Retinal Degeneration.
Invest. Ophthalmol. Vis. Sci. (2012) 22589437

Martino ME, Olsen JC, Fulcher NB, Wolfgang MC, O'Neal WK, Ribeiro CM.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
J. Biol. Chem. 284(22) 14904-13(2009) 19321437

Kamimura D, Bevan MJ.
Endoplasmic reticulum stress regulator XBP-1 contributes to effector CD8+ T cell differentiation during acute infection.
J. Immunol. 181(8) 5433-41(2008) 18832700

Bhootada Y, Kotla P, Zolotukhin S, Gorbatyuk O, Bebok Z, Athar M, Gorbatyuk M.
Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa.
PLoS ONE (2016) 27144303

Braun, M., Tatreau, J., Fulcher, N., Wolfgang, M., Randell, S., O eal, W., and Ribeiro, C. M. P.
Pseudomonas aeruginosa activates the unfolded protein response in airway epithelia: relevance for airway in inflammation.
Pediatric Pulmonology, Supplement 30 260(2007)