Strain Data Sheet

RBRC01084

Information1

Image
BRC No.RBRC01084
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Drd5<tm1Mok>
Former Common nameD5R KO, B6-D5R KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences.
Strain descriptionD5R (Drd5) gene knockout mice. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesPLoS One, 8(10):e75975 (2013). doi: 10.1371/journal.pone.0075975. 24155877

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Drd5dopamine receptor 55Drd5<tm1Mok>targeted mutation 1, Motoya KatsukiDrd-5, DRD1b, Drd1b, Gpcr1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EcogptE.coli xanthine phosphoribosyltransferase5Ecogptmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli xanthine phosphoribosyltransferase(Ecogpt), mouse Drd5 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLOS ONE, 8(10):e75975 (2013). doi: 10.1371/journal.pone.0075975.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshikuni Sasaoka (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Sasamori H, Asakura T, Sugiura C, Bouchekioua Y, Nishitani N, Sato M, Yoshida T, Yamasaki M, Terao A, Watanabe M, Ohmura Y, Yoshioka M.
Behavioral characteristics of dopamine D5 receptor knockout mice.
Sci Rep 12(1) 6014(2022) 35399112