Strain Data Sheet

RBRC01081

Information1

Image
BRC No.RBRC01081
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Drd2<tm1Mok>
Former Common nameD2R KO, B6-D2R KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearanceblack
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Medical Institute of Bioregulation, Kyushu University).
Strain descriptionD2R (Drd2) gene knockout mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesFront Cell Dev Biol., 3, 38 (2015). 26137459
Genes Cells, 1, 253-268 (1996). 9140068
Front Integr Neurosci., 8, 56 (2014). 25076876

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Drd2dopamine receptor D29Drd2<tm1Mok>targeted mutation 1, Motoya KatsukiD2 receptor, D2R, Drd-2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse dopamine receptor2 genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 1, 253-68, (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshikuni Sasaoka (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Shioda N, Yabuki Y, Wang Y, Uchigashima M, Hikida T, Sasaoka T, Mori H, Watanabe M, Sasahara M, Fukunaga K.
Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRβ signaling in striatopallidal medium spiny neurons.
Mol Psychiatry 22(8) 1205-1222(2017) 27922607

Wakabayashi C, Numakawa T, Ooshima Y, Hattori K, Kunugi H.
Possible role of the dopamine D1 receptor in the sensorimotor gating deficits induced by high-fat diet.
Psychopharmacology (Berl) 232(24) 4393-400(2015) 26359228