Strain Data Sheet

RBRC01081

Strain Information

Image
BRC No.RBRC01081
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Drd2<tm1Mok>
Former Common nameD2R KO, B6-D2R KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearanceblack
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Medical Institute of Bioregulation, Kyushu University).
Strain descriptionD2R (Drd2) gene knockout mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary.
Yamaguchi H, Aiba A, Nakamura K, Nakao K, Sakagami H, Goto K, Kondo H, Katsuki M
Genes Cells, 1, 253-268 (1996). 9140068

Distinct motor impairments of dopamine D1 and D2 receptor knockout mice revealed by three types of motor behavior.
Nakamura T, Sato A, Kitsukawa T, Momiyama T, Yamamori T, Sasaoka T
Front Integr Neurosci., 8, 56 (2014). 25076876

Expression pattern of immediate early genes in the cerebellum of D1R KO, D2R KO, and wild type mice under vestibular-controlled activity.
Nakamura T, Sato A, Kitsukawa T, Sasaoka T, Yamamori T
Front Cell Dev Biol., 3, 38 (2015). 26137459

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Drd2
MGI:94924
dopamine receptor D29Drd2<tm1Mok>
MGI:3576789
targeted mutation 1, Motoya Katsuki
neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circulating hormone level(MP:0005418)

  • abnormal single cell response(MP:0003463)

  • darkened coat color(MP:0005409)

  • enlarged pituitary intermediate lobe(MP:0008370)

  • increased pituitary hormone level(MP:0003973)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse dopamine receptor2 genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 1, 253-68, (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Shioda N, Yabuki Y, Wang Y, Uchigashima M, Hikida T, Sasaoka T, Mori H, Watanabe M, Sasahara M, Fukunaga K.
    Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRβ signaling in striatopallidal medium spiny neurons.
    Mol Psychiatry 22(8) 1205-1222(2017) 27922607
    Wakabayashi C, Numakawa T, Ooshima Y, Hattori K, Kunugi H.
    Possible role of the dopamine D1 receptor in the sensorimotor gating deficits induced by high-fat diet.
    Psychopharmacology (Berl) 232(24) 4393-400(2015) 26359228