Strain Data Sheet

RBRC01071

Information1

Image
BRC No.RBRC01071
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Rbpj<tm1Hon>/HonRbrc
Former Common nameFloxed RBP-J
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.
Strain descriptionRbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote. Homozygous mice are viable and fertile.
ReferencesInt. Immunol., 14, 637-645 (2002). 12039915

Health Report

Examination Date / Room / Rack2022/05/31Room:4-CRack:C
2022/03/15Room:4-CRack:C
2021/12/13Room:4-CRack:C
2021/09/13Room:4-CRack:C
2021/06/14Room:4-CRack:C
2021/03/29Room:4-CRack:C
2021/01/05Room:4-CRack:C
2020/10/05Room:4-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rbpjrecombining binding protein suppressor of hairless (Drosophila)5Rbpj<tm1Hon>targeted mutation 1, Tasuku HonjoRBPf, RBP-JC, RBP-Jf, Rbpjflox, Rbp-Jflox, RBP-JkappaFl, RBP-Jkappaflox, RBPJkappaloxP, RBP-Jlox, RbpjloxP, Rbpsuhtm3Kyo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, E. coli neo, mouse RBP-J genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCre/loxP system
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Int, Immunol., 14, 637-645 (2002).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Honjo Lab HP

BRC mice in Publications

Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis.
Dev Biol 332(2) 339-50(2009) 19501584

Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R.
The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock.
Dev Cell 13(2) 298-304(2007) 17681139

Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J.
In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation.
Dev Dyn 241(4) 684-96(2012) 22354878

Mead TJ, Yutzey KE.
Notch pathway regulation of neural crest cell development in vivo.
Dev Dyn 241(2) 376-89(2012) 22275227

Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ.
Notch signaling controls liver development by regulating biliary differentiation.
Development 136(10) 1727-39(2009) 19369401

Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H.
Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues.
EMBO Rep 15(12) 1297-304(2014) 25378482

Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.
O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals.
Elife 6 (2017) 28395734

Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y.
A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice.
J Immunol 194(1) 243-51(2015) 25429074

Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H.
Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.
J Immunol 188(5) 2427-36(2012) 22279105

Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T.
Regulation of myeloid leukaemia by the cell-fate determinant Musashi.
Nature 466(7307) 765-8(2010) 20639863

Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H.
Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development.
Proc Natl Acad Sci U S A 110(5) 1875-80(2013) 23319657