Strain Data Sheet
RBRC01071
Information1 | |
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| Image |  |
| BRC No. | RBRC01071 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Rbpj<tm1Hon>/HonRbrc |
| Former Common name | Floxed RBP-J |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6. |
| Strain description | Rbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice. |
| Colony maintenance | Homozygote x Homozygote. Homozygous mice are viable and fertile. |
| References | Int. Immunol., 14, 637-645 (2002). 12039915 |
Health Report | |
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| Examination Date / Room / Rack | 2023/08/28Room:4-CRack:C 2023/05/29Room:4-CRack:C 2023/02/27Room:4-CRack:C 2022/11/29Room:4-CRack:C 2022/08/29Room:4-CRack:C 2022/05/31Room:4-CRack:C 2022/03/15Room:4-CRack:C 2021/12/13Room:4-CRack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Rbpjrecombining binding protein suppressor of hairless (Drosophila)5Rbpj<tm1Hon>targeted mutation 1, Tasuku HonjoRBPf, RBP-JC, RBP-Jf, Rbpjflox, Rbp-Jflox, RBP-JkappaFl, RBP-Jkappaflox, RBPJkappaloxP, RBP-Jlox, RbpjloxP, Rbpsuhtm3Kyo Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | phage P1 loxP sites, E. coli neo, mouse RBP-J genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter) |
| Research application | Cre/loxP system |
| Specific Term and Conditions | 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Int, Immunol., 14, 637-645 (2002). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
| Depositor | Tasuku Honjo (Kyoto University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Genetic Background Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K. RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis. Dev Biol 332(2) 339-50(2009) 19501584 Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R. The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock. Dev Cell 13(2) 298-304(2007) 17681139 Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J. In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation. Dev Dyn 241(4) 684-96(2012) 22354878 Mead TJ, Yutzey KE. Notch pathway regulation of neural crest cell development in vivo. Dev Dyn 241(2) 376-89(2012) 22275227 Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ. Notch signaling controls liver development by regulating biliary differentiation. Development 136(10) 1727-39(2009) 19369401 Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H. Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues. EMBO Rep 15(12) 1297-304(2014) 25378482 Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T. O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals. Elife 6 (2017) 28395734 Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y. A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice. J Immunol 194(1) 243-51(2015) 25429074 Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H. Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis. J Immunol 188(5) 2427-36(2012) 22279105 Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature 466(7307) 765-8(2010) 20639863 Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H. Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development. Proc Natl Acad Sci U S A 110(5) 1875-80(2013) 23319657 |