BRC No.RBRC01071
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameFloxed RBP-J
Former Common nameFloxed RBP-J
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.
Strain descriptionRbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote. Homozygous mice are viable and fertile.
ReferencesInt. Immunol., 14, 637-645 (2002). 12039915

Health Report

Examination Date / Room / Rack2020/03/30Room:4-CRack:C


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rbpjrecombining binding protein suppressor of hairless (Drosophila)5Rbpj<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAphage P1 loxP sites, E. coli neo, mouse RBP-J genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCre/loxP system
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Int, Immunol., 14, 637-645 (2002).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis.
Dev. Biol. 332(2) 339-50(2009) 19501584

Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J.
In vivo notch reactivation in differentiating cochlear hair cells induces sox2 and prox1 expression but does not disrupt hair cell maturation.
Dev. Dyn. 241(4) 684-96(2012) 22354878

Mead TJ, Yutzey KE.
Notch pathway regulation of neural crest cell development in vivo.
Dev. Dyn. 241(2) 376-89(2012) 22275227

Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ.
Notch signaling controls liver development by regulating biliary differentiation.
Development 136(10) 1727-39(2009) 19369401

Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H.
Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues.
EMBO Rep. 15(12) 1297-304(2014) 25378482

Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.
O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals.
Elife 6 (2017) 28395734

Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y.
A Genome-Wide Analysis Identifies a Notch-RBP-J kappa-IL-7R alpha Axis That Controls IL-17-Producing gamma delta T Cell Homeostasis in Mice.
J. Immunol. (2014) 25429074

Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H.
Epithelial Cell-Intrinsic Notch Signaling Plays an Essential Role in the Maintenance of Gut Immune Homeostasis.
J. Immunol. (2012) 22279105

Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T.
Regulation of myeloid leukaemia by the cell-fate determinant Musashi.
Nature 466(7307) 765-8(2010) 20639863

Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H.
Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development.
Proc. Natl. Acad. Sci. U.S.A. (2013) 23319657