Strain Data Sheet



BRC No.RBRC01058
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nrp1<tm1Hfu>/HfuRbrc
Former Common nameICR-Nrp1<tmHfu>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR
Strain developmentDeveloped by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein. ICR background.
Strain descriptionICR.Cg-Nrp1<tm1Hfu>. Neuropilin-1 (Nrp1) is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D. Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote) or Cross to ICR (Heterozygote x Slc:ICR)Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11.
ReferencesNeuron, 19, 995-1005 (1997). 9390514

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nrp1neuropilin 18Nrp1<tm1Hfu>targeted mutation 1, Hajime FujisawaNeuropilin-1, NP-1, NPN-1, Npn1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)


Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 poly A, mouse C57BL/6 Neuropilin-1 genomic DNA
Research applicationCardiovascular Research
Developmental Biology Research
Hematological Research
Immunology and Inflammation Research
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997).
DepositorHajime Fujisawa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Hatanaka Y, Matsumoto T, Yanagawa Y, Fujisawa H, Murakami F, Masu M.
Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
J Comp Neurol 514(3) 215-25(2009) 19296474