Strain Data Sheet

RBRC01058

Information1

Image
BRC No.RBRC01058
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nrp1<tm1Hfu>/HfuRbrc
Former Common nameICR-Nrp1<tmHfu>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR
Appearancealbino
Strain developmentDeveloped by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein. ICR background.
Strain descriptionICR.Cg-Nrp1<tm1Hfu>. Neuropilin-1 (Nrp1) is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D. Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote) or Cross to ICR (Heterozygote x Slc:ICR)Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11.
ReferencesNeuron, 19, 995-1005 (1997). 9390514

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nrp1neuropilin 18Nrp1<tm1Hfu>targeted mutation 1, Hajime FujisawaNeuropilin-1, NP-1, NPN-1, Npn1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 poly A, mouse C57BL/6 Neuropilin-1 genomic DNA
Research applicationCardiovascular Research
Developmental Biology Research
Hematological Research
Immunology and Inflammation Research
Neurobiology Research
covid19_infection
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997).
DepositorHajime Fujisawa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Hatanaka Y, Matsumoto T, Yanagawa Y, Fujisawa H, Murakami F, Masu M.
Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
J Comp Neurol 514(3) 215-25(2009) 19296474