RBRC01058

Information1

Image
BRC No.RBRC01058
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR-Nrp1tmHfu
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR
Appearancealbino
Strain developmentDeveloped by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein. ICR background.
Strain descriptionICR.Cg-Nrp1<tm1Hfu>. Neuropilin-1 (Nrp1) is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D. Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote) or Cross to ICR (Heterozygote x Slc:ICR)Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11.
ReferencesNeuron, 19, 995-1005 (1997). 9390514

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nrp1neuropilin 18Nrp1<tm1Hfu>targeted mutation 1, Hajime Fujisawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse Neuropilin genomic DNA, E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997).
DepositorHajime Fujisawa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Hatanaka Y, Matsumoto T, Yanagawa Y, Fujisawa H, Murakami F, Masu M.
Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
J. Comp. Neurol. 514(3) 215-25(2009) 19296474