RBRC01046

Information1

Image
BRC No.RBRC01046
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Atf2<tm1Sis>/Rbrc
Former Common nameATF2o Knock out (CRE-BP1)-B6
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Toshio Maekawa and Shunsuke Ishii, RIKEN Tsukuba Institute in 1999.
Strain descriptionATF2 (CRE-BP1) is a transcription factor of the ATF/CREB family. This protein has a zinc finger structure as a transactivation domain located at the amino terminus and a leucin zipper motif, and phosphorylated by the stress-activated protein kinase such as the Jun amino-terminal kinase and p38. ATF2 deficient mice die shortly after birth and display symptoms of severe respiratory distress with lungs filled with meconium. This strain is useful as an animal model for meconium aspiration syndrome (MAS).
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesJ. Biol. Chem., 274, 17813-17819 (1999). 10364225

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Atf2activating transcription factor 22Atf2<tm1Sis>targeted mutation 1, Laurie Glimcher

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse activating transcription factor 2 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Maekawa, T., Bernier, F., Sato, M., Nomura, S., Singh, M., Inoue, Y., Tokunaga, T., Imai, H., Yokoyama, M., Reimold, A., Glimcher, L. H. & Ishii, S. Mouse ATF-2 null mutants display features of severe type of meconium aspiration syndrome. J. Biol. Chem., 274, 17813-17819 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 1. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
2. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
DepositorShunsuke Ishii (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data