Strain Data Sheet

RBRC01003

Strain Information

Image
BRC No.RBRC01003
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ambp<tm1Kkm>
Former Common nameBikunin, SHAP-Hyaluronic acid complex formation deficient mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6NCrSlc
Appearance
Strain development
Strain description
Colony maintenance
References
Defect in SHAP-hyaluronan complex causes severe female infertility. A study by inactivation of the bikunin gene in mice.
Zhuo L, Yoneda M, Zhao M, Yingsung W, Yoshida N, Kitagawa Y, Kawamura K, Suzuki T, Kimata K
J. Biol. Chem., 276, 7693-7696 (2001). 11145954

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 Late and early polyA signal4
Ambpalpha 1 microglobulin/bikunin precursor4Ambptargeted mutation 1, Koji Kimata
neoneomycin resistance gene (E. coli)4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal maternal nurturing(MP:0001386)

  • abnormal oocyte morphology(MP:0001125)

  • absent cumulus expansion(MP:0009374)

  • decreased litter size(MP:0001935)

  • decreased oocyte number(MP:0005431)
    • more 4 phenotypes
  • failure of zygotic cell division(MP:0003406)

  • female infertility(MP:0001926)

  • impaired fertilization(MP:0000242)

  • reduced female fertility(MP:0001923)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHSV thymidine kinase promoter, E coli Neomycin resistance gene, HSV thymidine kinase polyA signal, SV40 Late and early polyA signal (a 139bp fragment between HpaI and BamHI sites in pMAMneo-S vector(Clontech), mouse Ambp genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Biol Chem. 2001 276(11):7693-6RECIPIENT of BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by DEPOSITOR with DEPOSITOR prior to obtaining the BIOLOGICAL RESOURCE.
    DepositorLisheng Zhuo (Aichi Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. MATERIAL TRANSFER AGREEMENT (Pdf)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data