RBRC01000

Information1

Image
BRC No.RBRC01000
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Fyn<tm1Yik>/TyagRbrc
Former Common nameB6.Cg-Fyn<tm1Yik>, Fyn Targeting mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6 and CBA mixed
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Takeshi Yagi, Tsukuba Life Science Center, RIKEN in 1993. The targeting vector containing lacZ-neo cassette was transferred into TT2 ES cells to replace the SH1, SH3 and tyrosine kinase regions of the Fyn gene. The mutant mice were crossed to C57BL/6.
Strain descriptionFyn gene knockout mice. Fyn proto-oncogene is a non-receptor type tyrosine kinase of Src family that is expressed in the central nervous system and suggested to be involved in the mechanism of neuronal signaling cascade. Homozygous mutant mice exhibit a defect in suckling behavior and abnormal hippocampus morphology. This strain is useful for elucidation of the role of Fyn tyrosine kinase.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesScience, 278, 698-701 (1997). 9381182
Nature, 366, 742-745 (1993). 8264796

Health Report

Examination Date / Room / Rack2011/05/11Room:2-3Rack:C
2011/05/09Room:2-3Rack:C
2011/04/25Room:2-3Rack:C
2010/01/12Room:2-3Rack:C
2009/11/09Room:2-3Rack:C
2009/09/07Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FynFyn proto-oncogene10Fyn<tm1Yik>targeted mutation 1, Yoji Ikawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)10

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli lacZ, polyA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Fyn genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 366, 742-745 (1993).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.
DepositorTakeshi Yagi (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Nunomura S, Gon Y, Yoshimaru T, Kashiwakura J, Kawakami T, Ra C.
FcepsilonRI beta-chain ITAM amplifies PI3K-signaling to ensure synergistic degranulation response via FcepsilonRI and adenosine receptors.
Eur. J. Immunol. 40(4) 1205-17(2010) 20101614

Miyata N, Gon Y, Nunomura S, Endo D, Yamashita K, Matsumoto K, Hashimoto S, Ra C.
Inhibitory effects of parthenolide on antigen-induced microtubule formation and degranulation in mast cells.
Int. Immunopharmacol. 8(6) 874-80(2008) 18442792