Strain Data Sheet

RBRC01000

Strain Information

Image
BRC No.RBRC01000
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Fyn<tm1Yik>/TyagRbrc
Former Common nameB6.Cg-Fyn<tm1Yik>, Fyn Targeting mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6 and CBA mixed
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Takeshi Yagi, Tsukuba Life Science Center, RIKEN in 1993. The targeting vector containing lacZ-neo cassette was transferred into TT2 ES cells to replace the SH1, SH3 and tyrosine kinase regions of the Fyn gene. The mutant mice were crossed to C57BL/6.
Strain descriptionFyn gene knockout mice. Fyn proto-oncogene is a non-receptor type tyrosine kinase of Src family that is expressed in the central nervous system and suggested to be involved in the mechanism of neuronal signaling cascade. Homozygous mutant mice exhibit a defect in suckling behavior and abnormal hippocampus morphology. This strain is useful for elucidation of the role of Fyn tyrosine kinase.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References
A role for Fyn tyrosine kinase in the suckling behaviour of neonatal mice.
Yagi T, Aizawa S, Tokunaga T, Shigetani Y, Takeda N, Ikawa Y
Nature, 366, 742-745 (1993). 8264796

Fyn-kinase as a determinant of ethanol sensitivity: relation to NMDA-receptor function.
Miyakawa T, Yagi T, Kitazawa H, Yasuda M, Kawai N, Tsuboi K, Niki H
Science, 278, 698-701 (1997). 9381182

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FynFyn proto-oncogene10Fyntargeted mutation 1, Yoji Ikawa
lacZbeta-galactosidase (E. coli)10
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Sertoli cell barrier morphology(MP:0020355)

  • abnormal circadian behavior(MP:0020467)

  • abnormal cytokine secretion(MP:0003009)

  • abnormal excitatory postsynaptic potential(MP:0002912)

  • abnormal hippocampus layer morphology(MP:0000813)
    • more 27 phenotypes
  • abnormal hypothalamus morphology(MP:0000837)

  • abnormal interleukin secretion(MP:0008568)

  • abnormal locomotor circadian rhythm(MP:0020477)

  • abnormal maternal nurturing(MP:0001386)

  • abnormal myelination(MP:0000920)

  • abnormal olfaction(MP:0001984)

  • abnormal olfactory bulb morphology(MP:0000819)

  • abnormal pup retrieval(MP:0001384)

  • abnormal seminiferous tubule morphology(MP:0002216)

  • abnormal serotonin level(MP:0005322)

  • abnormal somatosensory cortex morphology(MP:0000859)

  • abnormal startle reflex(MP:0001486)

  • abnormal stratification in cerebral cortex(MP:0000790)

  • abnormal suckling behavior(MP:0001436)

  • audiogenic seizures(MP:0001496)

  • decreased testis weight(MP:0004852)

  • enhanced behavioral response to alcohol(MP:0009751)

  • enhanced passive avoidance behavior(MP:0003999)

  • increased dopamine level(MP:0001906)

  • increased fear-related response(MP:0003459)

  • increased serotonin level(MP:0010069)

  • increased susceptibility to experimental autoimmune uveoretinitis(MP:0004821)

  • increased susceptibility to xenobiotic induced morbidity/mortality(MP:0009766)

  • increased tumor necrosis factor secretion(MP:0008560)

  • oligozoospermia(MP:0002687)

  • prolonged circadian behavior period(MP:0020469)

  • small seminiferous tubules(MP:0001153)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, polyA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Fyn genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 366, 742-745 (1993).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
    When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.
    DepositorTakeshi Yagi (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Nunomura S, Gon Y, Yoshimaru T, Kashiwakura J, Kawakami T, Ra C.
    FcepsilonRI beta-chain ITAM amplifies PI3K-signaling to ensure synergistic degranulation response via FcepsilonRI and adenosine receptors.
    Eur J Immunol 40(4) 1205-17(2010) 20101614
    Miyata N, Gon Y, Nunomura S, Endo D, Yamashita K, Matsumoto K, Hashimoto S, Ra C.
    Inhibitory effects of parthenolide on antigen-induced microtubule formation and degranulation in mast cells.
    Int Immunopharmacol 8(6) 874-80(2008) 18442792