RBRC00995

Information1

Image
BRC No.RBRC00995
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Il5ra<tm1Ktk>/KtkRbrc
Former Common nameC57BL/6 IL-5R alpha -/-
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6CrSlc.
Strain descriptionL-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
ReferencesImmunity, 4, 483-494 (1996). 8630733
Immunology, 102, 289-300 (2001). 11298827

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Il5rainterleukin 5 receptor, alpha6Il5ra<tm1Ktk>targeted mutation 1, Kiyoshi Takatsu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IL-5R α genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 4, 483-494 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided.
Users should contact the depositor in the case of application for any patents with the results from these mice.
DepositorKiyoshi Takatsu (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data