RBRC00984

Information1

Image
BRC No.RBRC00984
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129P2-Nfe2l2<tm1Mym>/MymRbrc
Former Common nameNrf2 knock out mouse (ICR background)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainJcl:ICR
Appearance
Strain developmentDeveloped by Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 1996. The targeting vector containing lacZ-neo cassette was transferred into E14 ES cells to replace the 1.2 kb segment containing the rest of the exon 5 coding sequence of the Nrf2 gene.
Strain descriptionNrf2 (Nfe2l2) Knockout mice. This strain is a useful model for the in vivo analysis of chemical carcinogenesis and resistance to anti-cancer drugs. A phenolic antioxidant significantly induced the expression of phase II enzymes such as glutathione S-transferase and NAD(P)H: quinone oxidoreductase in normal mice. However, in the homozygous Nrf2 deficient mice the induction of the phase II enzymes by a phenolic antioxidant was largely eliminated in the liver and intestine. Since Nrf2 is constantly degraded by proteasome under normal condition, it is hardly to detect Nrf2 protein in the absence of stimuli. Electrophilic chemicals, such as DEM (diethyl maleate) and tBHQ (tert-buthyl hydroquinone), are generally used to stabilize Nrf2. Or proteasomal inhibitors, such as MG132, are also effective increasing the protein amount of Nrf2. C57BL/6 background (RBRC01390), BALB/c background (RBRC02190), A/J background (RBRC02414), ICR mixed background (RBRC00984). Homozygous mutant mice are viable and fertile, but show lower reproductive performance.
Colony maintenanceHomozygote x Heterozygote [or Crossing to Jcl:ICR]
ReferencesBiochem. Biophys. Res. Commun., 236, 313-322 (1997). 9240432

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nfe2l2nuclear factor, erythroid derived 2, like 22Nfe2l2<tm1Mym>targeted mutation 1, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)2nls

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Nrf2 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 236, 313-322 (1997). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 2 year after provided.
Any patent rights and other intellectual properties are retained by the depositor. Users should contact the depositor in the case of application for any patents with the results from these mice.
DepositorMasayuki Yamamoto (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Oct 2005
Mouse of the Month Apr 2012

BRC mice in Publications

Ramkissoon A, Wells PG.
Methamphetamine Oxidative Stress, Neurotoxicity, And Functional Deficits Are Modulated By Nuclear Factor-E2-Related Factor 2.
Free Radic. Biol. Med. (2015) 26427884

Ramkissoon A, Wells PG.
Developmental role of nuclear factor E2-related factor 2 in mitigating methamphetamine fetal toxicity and postnatal neurodevelopmental deficits.
Free Radic. Biol. Med. 65C 620-631(2013) 23932974

Ho E, Clarke JD, Dashwood RH.
Dietary sulforaphane, a histone deacetylase inhibitor for cancer prevention.
J. Nutr. 139(12) 178756(2009) 19812222

Clarke JD, Hsu A, Williams DE, Dashwood RH, Stevens JF, Yamamoto M, Ho E.
Metabolism and Tissue Distribution of Sulforaphane in Nrf2 Knockout and Wild-Type Mice.
Pharm. Res. (2011) 21681606

Gebel S, Diehl S, Pype J, Friedrichs B, Weiler H, Sch ller J, Xu H, Taguchi K, Yamamoto M, M ller T.
The transcriptome of Nrf2-/- mice provides evidence for impaired cell cycle progression in the development of cigarette smoke-induced emphysematous changes.
Toxicol. Sci. 115(1) 238-52(2010) 20133372