RBRC00983

Information1

Image
BRC No.RBRC00983
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gata2<tm1Mym>/Rbrc
Former Common nameGATA-2 2nd Exon GFP knock in
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Naoko Minegishi and Masayuki Yamamoto, Center for Tukuba Advanced Research Alliance, University of Tsukuba in 2003. GFP was inserted in exon 2 of the Gata2 gene (Blood, 2003).
Strain descriptionB6;129P2-Gata2<tm1Mym>. GFP knock-in mice in the Gata2 locus, replacing the endogenous Gata2 coding sequences in the second exon. GFP expression was observed in the early hematopoietic tissues. Homozygous mutat mice are embryonic lethal (E11.0).
Colony maintenanceHeterozygote x Wild-type [C57BL/6J]Homozygous mutat mice are embryonic lethal (E11.0).
ReferencesBlood, 102, 896-905 (2003). 12689939

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gata2GATA binding protein 26Gata2<tm1Mym>targeted mutation 1, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)6

Information2

Donor DNAJellyfish GFP cDNA, mouse GATA-2 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in articles when the users publish their data using these mice for 2 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice.
DepositorMasayuki Yamamoto (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

No Data