|Former Common name||152A; B6C3F1B6C3F1-TgN(act-EGFP)OsbCX-FM038|
|ES Cell line|
|Appearance||black or agouti [a/a B/B C/C or A/? B/B C/C]|
|Strain development||Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.|
|Strain description||The CAG-EGFP transgene is located on X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.|
|Colony maintenance||Sibling Mating|
|References||Genomics, 80, 564-574 (2002). 12504848|
FEBS Lett., 407, 313-319 (1997). 9175875
Curr. Biol., 16(2):166-172 (2006). 16431368
|Examination Date / Room / Rack||2006/06/19Room:3-DRack:F|
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)XCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
|Donor DNA||CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA|
|Research application||Fluorescent Proteins/lacZ System|
|Specific Term and Conditions||The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Curr. Biol., 16(2):166-172 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.|
|Depositor||Masaru Okabe (Osaka University)|
|Strain Status||Frozen embryos|
|Strain Availability||Recovered litters from cryopreserved embryos (2 to 4 months)|
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
|Additional Info.||Genotyping protocol -PCR-|
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
BRC mice in Publications
Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M.
The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos.
Nucleic Acids Res. 38(11) 3672-81(2010) 20185572
Watanabe-Takano H, Takano K, Sakamoto A, Matsumoto K, Tokuhisa T, Endo T, Hatano M.
DA-Raf-dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation.
Proc. Natl. Acad. Sci. U.S.A. (2014) 24843139