Strain Data Sheet



BRC No.RBRC00980
SpeciesMus musculus
Strain nameB6C3F1-Tg(CAG-EGFP)CX-FM038Osb
Former Common name152A; B6C3F1B6C3F1-TgN(act-EGFP)OsbCX-FM038
H-2 Haplotype
ES Cell line
Background strainSlc:B6C3F1
Appearanceblack or agouti [a/a B/B C/C or A/? B/B C/C]
Strain developmentDeveloped by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.
Strain descriptionThe CAG-EGFP transgene is located on X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.
Colony maintenanceSibling Mating
ReferencesFEBS Lett., 407, 313-319 (1997). 9175875
Genomics, 80, 564-574 (2002). 12504848
Curr. Biol., 16(2):166-172 (2006). 16431368

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPGreen Fluorescent Protein (Aequorea victoria)XEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)


Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Curr. Biol., 16(2):166-172 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University ( The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M.
The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos.
Nucleic Acids Res 38(11) 3672-81(2010) 20185572

Watanabe-Takano H, Takano K, Sakamoto A, Matsumoto K, Tokuhisa T, Endo T, Hatano M.
DA-Raf-dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation.
Proc Natl Acad Sci U S A 111(22) E2291-300(2014) 24843139