Strain Data Sheet

RBRC00961

Information1

Image
BRC No.RBRC00961
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gal3st1<tm1Kho>/KhoRbrc
Former Common nameC57BL/6N x CST KO-1(hetero), TgH(CSTneo) 01
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Koichi Honke, Osaka University Medical School in 1999. A targeting vector including pgk-neo was transfected into E14.1 ES cells to disrupt exons 2 and 3 of Cst gene. The Cst knockout mice were backcrossed to C57BL/6N for 6 times. Generation: N5+N1F3
Strain descriptionB6;129P2-Gal3st1<tm1Kho>. Cerebroside sulfotransferase (Cst) gene is located in the Golgi membrane that is responsible for the biosynthesis of two sulfated glycolipids (GalCer and GalEAG) in the mammals. Cst null mice lack sulfatide in myelin and sulfated seminolipid in sperm. Homozygous mutant males are sterile. This strain is useful for elucidation of the role of sulfoglycolipids in myelin function and spermatogenesis.
Colony maintenance
ReferencesJ. Neurosci., 22, 6507-6514 (2002). 12151530
J. Biol. Chem., 279, 2085-2090 (2004). 14583626
Glia, 45, 269-277 (2004). 14730700
Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). 11917099

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gal3st1galactosylceramide sulfotransferase11Gal3st1<tm1Kho>targeted mutation 1, Koichi Honke3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase, CST, galactosylceramide sulfotransferase, GalCer sulfotransferase

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Gal3st1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002).
DepositorKoichi Honke (Kochi University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Zhang Y, Hayashi Y, Cheng X, Watanabe T, Wang X, Taniguchi N, Honke K.
Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis.
Glycobiology 15(6) 649-54(2005) 15659616