Strain Data Sheet

RBRC00915

Information1

Image
BRC No.RBRC00915
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.Cg-Irf9<tm1Ttg>/TtgRbrc
Former Common nameIRF-9 KO/BALB/c; BALB/cAJcl-Isgf3g<tm1>, IRF-9 Knockout mouse, BALB/c
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cAJcl
Appearancealbino
Strain developmentDeveloped by Tadatsugu Taniguchi, Graduate School of Medicine and Faculty of Medicine, University of Tokyo. RBRC00915: Backcrossed to BALB/c (N8 generations), RBRC00916: Backcrossed to C57BL/6J (over N13 generations).
Strain descriptionIRF-9 gene knockout mice. Homozygous mutant mice exhibit lower serum interferon levels in response to viral infection. BALB/c background (RBRC00915), C57BL/6 background (RBRC00916).
Colony maintenanceHomozygote x Homozygote
ReferencesGenes Cells, 1, 115-124 (1996). 9078371

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Irf9interferon regulatory factor 914Irf9<tm1Ttg>targeted mutation 1, Tadatsugu TaniguchiIrf-9, p48, Isgf3g

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse IRF-9 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE and shall limit the use within the scope of the purpose described in this Agreement. The RECIPIENT shall inform the DEPOSITOR about the results obtained by the use of the BIOLOGICAL RESOURCE.
DepositorTadatsugu Taniguchi (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Mouse of the Month May 2005
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Thibault DL, Chu AD, Graham KL, Balboni I, Lee LY, Kohlmoos C, Landrigan A, Higgins JP, Tibshirani R, Utz PJ.
IRF9 and STAT1 are required for IgG autoantibody production and B cell expression of TLR7 in mice.
J Clin Invest 118(4) 1417-26(2008) 18340381

Martinet V, Tonon S, Torres D, Azouz A, Nguyen M, Kohler A, Flamand V, Mao CA, Klein WH, Leo O, Goriely S.
Type I interferons regulate eomesodermin expression and the development of unconventional memory CD8(+) T cells.
Nat Commun 6 7089(2015) 25953241