RBRC00906

Information1

Image
BRC No.RBRC00906
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(pLck-EGFP)1Naka/Rbrc
Former Common nameplck-GFP Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [(a/a B/B C/C)]
Strain developmentDeveloped by Dr. Toshinori Nakayama, Graduate School of Medicine, Chiba University in 2001. The 4.2 kb pLck-EGFP plasmid was purified and injected into the pronucleous of C57BL/6J fertilized eggs. Transgenic mice were backcrossed to C57BL/6 for 4 times.
Strain descriptionC57BL/6-Tg(pLck-EGFP)1Naka. The plck-GFP transgenic mice carry EGFP (enhanced green fluorescence protein) cDNA under control of the proximal promoter of Lck (lymphocyte protein tryosine kinase). The Lck proximal promoter is reported to be most active in immature thymocytes. These transgenic mice are powerful tools for the visualization of the intracellular machinery that is crucial for the development of lymphocytes.
Colony maintenanceBackcross to C57BL/6J (Hemizygote x C57BL/6JJcl).Reproductive performance is good.
ReferencesInt. Immunol., 13, 105-117 (2001). 11133839

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNlck proximal promoter, human growth hormone enhancer

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (human)UNGH

Information2

Donor DNAproximal lck promoter, Jellyfish GFP cDNA, human GH enhancer
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 13, (1) 105-117 (2001). The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshinori Nakayama (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data