Strain Data Sheet

RBRC00905

Information1

Image
BRC No.RBRC00905
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(dLck-GFP)1Naka/Rbrc
Former Common nameC57BL/6J-Tg(dLck-EGFP), dlck-GFP Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Nakayama T, Dept. Mol. Immunol., Graduate Sch. Med., Chiba Univ.
Strain descriptionThis transgenic mice express green fluorescence protein (GFP) under the control of the distal promotor of Lck.
Colony maintenanceHemizygotes x C57BL/6. Transgenic mice may breed as well as wild-type mice.
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNlck distal promoter, human growth hormone enhancer

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)UNGH

Information2

Donor DNAdistal lck promoter, Jellyfish GFP cDNA, human GH enhancer
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. As this BIOLOGICAL RESOURCE has been unpublished yet, the recipient is requested to use the BIOLOGICAL RESOURCE as collaborative study with Nakayama Toshinori, and the first publication should include Nakayama Toshinori as a co-author.
DepositorToshinori Nakayama (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

Yamashita M, Ukai-Tadenuma M, Miyamoto T, Sugaya K, Hosokawa H, Hasegawa A, Kimura M, Taniguchi M, DeGregori J, Nakayama T.
Essential role of GATA3 for the maintenance of type 2 helper T (Th2) cytokine production and chromatin remodeling at the Th2 cytokine gene loci.
J Biol Chem 279(26) 26983-90(2004) 15087456