RBRC00905

Information1

Image
BRC No.RBRC00905
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(dLck-GFP)1Naka/Rbrc
Former Common nameC57BL/6J-Tg(dLck-EGFP), dlck-GFP Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Nakayama T, Dept. Mol. Immunol., Graduate Sch. Med., Chiba Univ.
Strain descriptionThis transgenic mice express green fluorescence protein (GFP) under the control of the distal promotor of Lck.
Colony maintenanceHemizygotes x C57BL/6. Transgenic mice may breed as well as wild-type mice.
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNlck distal promoter, human growth hormone enhancer

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (human)UNGH

Information2

Donor DNAdistal lck promoter, Jellyfish GFP cDNA, human GH enhancer
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. As this BIOLOGICAL RESOURCE has been unpublished yet, the recipient is requested to use the BIOLOGICAL RESOURCE as collaborative study with Nakayama Toshinori, and the first publication should include Nakayama Toshinori as a co-author.
DepositorToshinori Nakayama (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

Masakatsu YAMASHITA, Maki UKAI-TADENUMA, Takeshi MIYAMOTO, Kaoru SUGAYA, Hiroyuki HOSOKAWA, Akihiro HASEGAWA, Motoko KIMURA, Masaru TANIGUCHI, James DEGREGORI, Toshinori NAKAYAMA
Essential role of GATA3 for the maintenance of type 2 helper T (Th2) cytokine production and chromatin remodeling at the Th2 cytokine gene loci.
J. Biol. Chem. 279 26983-26990(2004) 15087456