Strain Data Sheet

RBRC00904

Strain Information

Image
BRC No.RBRC00904
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)
Former Common namePD-1-KO-N12 (BALB/c)
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cCrSlc
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Tasuku Honjo, Kyoto University.
Strain descriptionThe PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and the immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygous mutant mice are fertile and viable.
References
Immunological studies on PD-1 deficient mice: implication of PD-1 as a negative regulator for B cell responses.
Nishimura H, Minato N, Nakano T, Honjo T
Int. Immunol., 10, 1563-1572 (1998). 9796923

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Nishimura H, Nose M, Hiai H, Minato N, Honjo T
Immunity, 11, 141-151 (1999). 10485649

Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice.
Nishimura H, Okazaki T, Tanaka Y, Nakatani K, Hara M, Matsumori A, Sasayama S, Mizoguchi A, Hiai H, Minato N, Honjo T
Science, 291, 319-322 (2001). 11209085

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pdcd1
MGI:104879		programmed cell death 11Pdcd1<tm1Hon>
MGI:2386184		targeted mutation 1, Tasuku Honjo
  • multiple sclerosis, susceptibility to(MedGEN)

  • systemic lupus erythematosus, susceptibility to, 2(MedGEN)
    • neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell activation(MP:0001828)

  • abnormal cardiac muscle contractility(MP:0002972)

  • abnormal cardiac output(MP:0001627)

  • abnormal cardiovascular system physiology(MP:0001544)

  • abnormal heart morphology(MP:0000266)
    • more 28 phenotypes
  • abnormal immunoglobulin level(MP:0002490)

  • abnormal leukopoiesis(MP:0005460)

  • abnormal myelopoiesis(MP:0001601)

  • abnormal response to transplant(MP:0005671)

  • arthritis(MP:0002993)

  • cardiac fibrosis(MP:0003141)

  • dilated heart ventricle(MP:0008022)

  • enlarged heart(MP:0000274)

  • enlarged liver(MP:0000599)

  • enlarged spleen(MP:0000691)

  • exophthalmos(MP:0002750)

  • glomerulonephritis(MP:0002743)

  • hepatic necrosis(MP:0001654)

  • increased B cell number(MP:0005014)

  • increased B cell proliferation(MP:0005154)

  • increased B-1 B cell number(MP:0004977)

  • increased IgA level(MP:0002495)

  • increased IgG2b level(MP:0008501)

  • increased IgG3 level(MP:0008502)

  • increased IgM level(MP:0002494)

  • increased autoantibody level(MP:0003725)

  • increased inflammatory response(MP:0001846)

  • increased interferon-gamma secretion(MP:0008566)

  • increased lymphocyte cell number(MP:0005013)

  • pancreas necrosis(MP:0009148)

  • premature death(MP:0002083)

  • pulmonary necrosis(MP:0010857)

  • thin ventricular wall(MP:0000280)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Mar 2005

    BRC mice in Publications

    Ding ZC, Lu X, Yu M, Lemos H, Huang L, Chandler P, Liu K, Walters M, Krasinski A, Mack M, Blazar BR, Mellor AL, Munn DH, Zhou G.
    Immunosuppressive myeloid cells induced by chemotherapy attenuate antitumor CD4+ T-cell responses through the PD-1-PD-L1 axis.
    Cancer Res 74(13) 3441-53(2014) 24780756
    Ding ZC, Huang L, Blazar BR, Yagita H, Mellor AL, Munn DH, Zhou G.
    Polyfunctional CD4⁺ T cells are essential for eradicating advanced B-cell lymphoma after chemotherapy.
    Blood 120(11) 2229-39(2012) 22859605
    Ding ZC, Blazar BR, Mellor AL, Munn DH, Zhou G.
    Chemotherapy rescues tumor-driven aberrant CD4+ T-cell differentiation and restores an activated polyfunctional helper phenotype.
    Blood 115(12) 2397-406(2010) 20118405