Strain Data Sheet


Strain Information

BRC No.RBRC00904
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)
Former Common namePD-1-KO-N12 (BALB/c)
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cCrSlc
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Tasuku Honjo, Kyoto University.
Strain descriptionThe PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and the immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygous mutant mice are fertile and viable.
ReferencesInt. Immunol., 10, 1563-1572 (1998). 9796923
Science, 291, 319-322 (2001). 11209085
Immunity, 11, 141-151 (1999). 10485649

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pdcd1programmed cell death 11Pdcd1<tm1Hon>targeted mutation 1, Tasuku HonjoLy101, PD-1, Pdc1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Honjo Lab HP
Genotyping protocol -PCR-
Mouse of the Month Mar 2005

BRC mice in Publications

Ding ZC, Huang L, Blazar BR, Yagita H, Mellor AL, Munn DH, Zhou G.
Polyfunctional CD4⁺ T cells are essential for eradicating advanced B-cell lymphoma after chemotherapy.
Blood 120(11) 2229-39(2012) 22859605

Ding ZC, Blazar BR, Mellor AL, Munn DH, Zhou G.
Chemotherapy rescues tumor-driven aberrant CD4+ T-cell differentiation and restores an activated polyfunctional helper phenotype.
Blood 115(12) 2397-406(2010) 20118405

Ding ZC, Lu X, Yu M, Lemos H, Huang L, Chandler P, Liu K, Walters M, Krasinski A, Mack M, Blazar BR, Mellor AL, Munn DH, Zhou G.
Immunosuppressive myeloid cells induced by chemotherapy attenuate antitumor CD4+ T-cell responses through the PD-1-PD-L1 axis.
Cancer Res 74(13) 3441-53(2014) 24780756