Strain Data Sheet
RBRC00904
Information1 | |
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| Image | |
| BRC No. | RBRC00904 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12) |
| Former Common name | PD-1-KO-N12 (BALB/c) |
| H-2 Haplotype | |
| ES Cell line | D3 [129S2/SvPas] |
| Background strain | BALB/cCrSlc |
| Appearance | albino [A/A b/b c/c] |
| Strain development | Developed by Dr. Tasuku Honjo, Kyoto University. |
| Strain description | The PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and the immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line. |
| Colony maintenance | Sibling Mating (Homozygote x Homozygote)Homozygous mutant mice are fertile and viable. |
| References | Int. Immunol., 10, 1563-1572 (1998). 9796923 Science, 291, 319-322 (2001). 11209085 Immunity, 11, 141-151 (1999). 10485649 |
Health Report | |
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| Examination Date / Room / Rack | 2022/07/11Room:3-ARack:F 2022/04/11Room:3-ARack:F 2022/01/24Room:3-ARack:F 2021/10/26Room:3-ARack:F 2021/07/26Room:3-ARack:F |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA |
| Research application | Immunology and Inflammation Research |
| Specific Term and Conditions | 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
| Depositor | Tasuku Honjo (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Mouse of the Month Mar 2005 Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Ding ZC, Huang L, Blazar BR, Yagita H, Mellor AL, Munn DH, Zhou G. Polyfunctional CD4⁺ T cells are essential for eradicating advanced B-cell lymphoma after chemotherapy. Blood 120(11) 2229-39(2012) 22859605 Ding ZC, Blazar BR, Mellor AL, Munn DH, Zhou G. Chemotherapy rescues tumor-driven aberrant CD4+ T-cell differentiation and restores an activated polyfunctional helper phenotype. Blood 115(12) 2397-406(2010) 20118405 Ding ZC, Lu X, Yu M, Lemos H, Huang L, Chandler P, Liu K, Walters M, Krasinski A, Mack M, Blazar BR, Mellor AL, Munn DH, Zhou G. Immunosuppressive myeloid cells induced by chemotherapy attenuate antitumor CD4+ T-cell responses through the PD-1-PD-L1 axis. Cancer Res 74(13) 3441-53(2014) 24780756 |