RBRC00897

Information1

Image
BRC No.RBRC00897
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
Former Common nameAID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
AppearanceBlack [a/a B/B C/C]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionAID (Aicda, activation-induced cytidine deaminase) knockout mice. Exon 2 and part of exon 3 were replaced with a neomycin resistance cassette. Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cell is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene was a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also indicated that somatic hypermutation of an immunoglobulin variable region gene and gene conversion do not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell.
Colony maintenanceHomozygote x Homozygote. Homozygous mutant mice are viable and fertile.
ReferencesCell, 102, 553-563 (2000). 11007474

Health Report

Examination Date / Room / Rack2020/06/01Room:3-ARack:I
2020/02/17Room:3-ARack:I
2019/12/16Room:3-ARack:I
2019/10/21Room:3-ARack:I
2019/08/26Room:3-ARack:I
2019/06/17Room:3-ARack:I
2019/04/15Room:3-ARack:I
2019/02/18Room:3-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Aicdaactivation-induced cytidine deaminase6Aicda<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Cell 102, 553-563 (2000).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (within 1 month)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-
Mouse of the Month Dec 2005
Genetic Background

BRC mice in Publications

Matsumoto M, Sasaki Y, Yasuda K, Takai T, Muramatsu M, Yoshimoto T, Nakanishi K.
IgG and IgE Collaboratively Accelerate Expulsion of Strongyloides venezuelensis in a Primary Infection.
Infect. Immun. 81(7) 2518-27(2013) 23630966

Kato M, Tsuji-Kawahara S, Kawasaki Y, Kinoshita S, Chikaishi T, Takamura S, Fujisawa M, Kawada A, Miyazawa M.
Class switch recombination and somatic hypermutation of virus-neutralizing antibodies are not essential for control of friend retrovirus infection.
J. Virol. 89(2) 1468-73(2014) 25378499