RBRC00892

Information1

Image
BRC No.RBRC00892
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-EGFP,-Aicda)20Hon/HonRbrc
Former Common nameAID-conditional Transgenic mouse line 20
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Tasuku Honjo and Ilmi Okazaki at Kyoto University in 2002.
Strain descriptionThese transgenic mice constitutively express GFP under control of CAG promoter. When crossed with a Cre recombinase-expressing strain, GFP expression is replaced with mouse AID expression in tissue expressing Cre. AID, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation, playing a central role in acquisition for B cell of antibody gene variety.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
ReferencesProc. Natl. Acad. Sci. USA, 103, 2752-2757 (2006). 16477013

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Aicdaactivation-induced cytidine deaminaseUNAicda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Jellyfish GFP cDNA, mouse Aicda cDNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 103, 2752-2757 (2006).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Honjo Lab HP

BRC mice in Publications

No Data